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anti-Mouse (Murine) PAG1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PAG1 Antibody (supplied by: Log in to see )

Antigen
phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
  • 8430414N03Rik
  • CBP
  • Cbp
  • F730007C19Rik
  • IGFBP-4ase
  • MGC80192
  • PAG
  • Pag
  • PAG1
  • PAPP-A
Epitope
C-Term
35
22
13
3
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
92
50
41
23
Host
Mouse
57
39
Clonality
Monoclonal
Conjugate
This PAG1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
71
56
30
23
21
11
3
1
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Catalog No. ABIN94270
$ 288.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008982679 ABIN372975 IHC (p) IP WB Rabbit AA 97-432 Log in to see Polyclonal
0.0008982679 ABIN120704 IHC (p) IP Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN94497 IHC (p) IP WB Rabbit IgG AA 97-432 Log in to see Polyclonal
0.0008982679 ABIN1723396 IHC (p) IP Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN121943 IHC (p) IP WB Rabbit AA 97-432 Log in to see Polyclonal
0.0008982679 ABIN1737819 IHC (p) IP WB Rabbit IgG AA 97-432 Log in to see Polyclonal
0.0008982679 ABIN269119 IHC (p) IP IHC WB Mouse IgG2b C-Term Log in to see PAG-C1 1
0.0008982679 ABIN269172 IHC (p) IP IHC WB Rabbit IgG AA 97-432 Log in to see Polyclonal
0.0008982679 ABIN4343411 IHC (p) WB Alexa Fluor 405 Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343412 IHC (p) WB Alexa Fluor 488 Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343413 IHC (p) WB Alexa Fluor 647 Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343414 IHC (p) WB Alexa Fluor 700 Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343415 IHC (p) WB Allophycocyanin Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343416 IHC (p) WB Biotin Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343417 IHC (p) WB DyLight 650 Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343418 IHC (p) WB FITC Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343419 IHC (p) WB DyLight 680 Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343420 IHC (p) WB DyLight 488 Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343422 IHC (p) WB DyLight 755 Mouse IgG2b C-Term Log in to see PAG-C1
0.0008982679 ABIN4343423 IHC (p) WB PerCP Mouse IgG2b C-Term Log in to see PAG-C1

Top referenced anti-PAG1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-PAG1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine)
Western Blotting (WB) 72 Antibodies 36 Antibodies 26 Antibodies 21 Antibodies
Immunoprecipitation (IP) 31 Antibodies 16 Antibodies 9 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies 32 Antibodies 26 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 24 Antibodies 2 Antibodies 4 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 3 Antibodies

General

Antigen phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
Epitope C-Term
(35), (22), (13), (3), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
(92), (50), (41), (23)
Host Mouse
(57), (39)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PAG1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(71), (56), (30), (23), (21), (11), (3), (1)
Pubmed 5 references available
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Product Details anti-PAG1 Antibody

Target Details PAG1 Application Details Handling References for anti-PAG1 Antibody (ABIN94270) Images
Specificity The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Clone PAG-C1
Isotype IgG2b

Target Details PAG1

Product Details anti-PAG1 Antibody Application Details Handling References for anti-PAG1 Antibody (ABIN94270) Images back to top
Antigen
Alternative Name PAG1 (PAG1 Antibody Abstract)
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Pathways p53 Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-PAG1 Antibody Target Details PAG1 Handling References for anti-PAG1 Antibody (ABIN94270) Images back to top
Application Notes Immunoprecipitation:
Positive control: RAJI human Burkitt lymphoma cell line

Immunohistochemistry (paraffin sections)
Positive tissue: Appendix (germinal center of lymphatic follicle
Application note: heat-mediated antigen retrieval in citrate buffer pH 6.1

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details References for anti-PAG1 Antibody (ABIN94270) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-PAG1 Antibody (ABIN94270)

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling Images back to top
Background publications

Simeoni, Smida, Posevitz, Schraven, Lindquist: "Right time, right place: the organization of membrane proximal signaling." in: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004

Baumgartner, Angelisová, Setterblad, Mooney, Werling, Horejsí, Langsley: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." in: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003

Product cited in:

Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003

Vang, Abrahamsen, Myklebust, Horejsí, Taskén: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003

Images

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling References for anti-PAG1 Antibody (ABIN94270) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-PAG1 antibody (phosphoprotein Associated with Glycosphingolipid Microdomains 1) (C-Term) (ABIN94270) Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma c...
Immunohistochemistry (IHC) image for anti-PAG1 antibody (phosphoprotein Associated with Glycosphingolipid Microdomains 1) (C-Term) (ABIN94270) Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of ly...