anti-Rat (Rattus) PAG1 antibody for Immunocytochemistry

Recommended PAG1 Antibody (supplied by: Log in to see )

Antigen
phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
  • MGC80192
  • CBP
  • PAG
  • Cbp
  • F730007C19Rik
  • Pag
  • 8430414N03Rik
  • IGFBP-4ase
  • PAG1
  • PAPP-A
  • phosphoprotein associated with glycosphingolipid microdomains 1
  • pregnancy-associated plasma protein A
  • PAG1
  • pag1
  • Pag1
  • Pappa
Epitope
AA 412-424, N-Term
36
22
16
2
2
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
97
63
48
29
2
2
1
1
1
Host
Rabbit
58
44
Clonality
Polyclonal
Conjugate
This PAG1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
72
56
39
33
21
13
5
3
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN2963729
$ 854.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2582424 ICC IP WB Rabbit AA 412-424 Log in to see Polyclonal
1 ABIN2745550 ICC IP WB Rabbit AA 412-424, N-Term Log in to see Polyclonal
1 ABIN5014124 ICC IHC IP WB Rabbit IgG AA 138-357 Log in to see Polyclonal

Similar anti-PAG1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 1 Antibodies 2 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies 4 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 18 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies 35 Antibodies
Immunoprecipitation (IP) 14 Antibodies 25 Antibodies
Western Blotting (WB) 27 Antibodies 42 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Flow Cytometry (FACS) 21 Antibodies

General

Antigen phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
Epitope AA 412-424, N-Term
(36), (22), (16), (2), (2), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(97), (63), (48), (29), (2), (2), (1), (1), (1)
Host Rabbit
(58), (44)
Clonality
Polyclonal
Conjugate This PAG1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(72), (56), (39), (33), (21), (13), (5), (3), (2), (1), (1)
Supplier Log in to see

Product Details anti-PAG1 Antibody

Target Details PAG1 Application Details Handling Images
Specificity Reacts with mouse PAG/Cbp
Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) Cross-reacts with rat and human protein.
Purification Antigen affinity purified
Immunogen Synthetic peptide corresponding to aa 412-424 of the N-terminal rat PAG including an additional N-terminal Cys residue.

Target Details PAG1

Product Details anti-PAG1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PAG/Cbp (PAG1 Antibody Abstract)
Background PAG [Cbp] is a ubiquitously expressed transmembrane adapter protein. Depending on its phosphorylation status, it appears as diffuse band(s) of 80-90 kDa by SDS-PAGE, with phosphorylation leading to a shift towards a higher apparent molecular mass. Tyrosine phosphorylated PAG recruits Csk to the membrane and negatively regulates Src family kinases via Csk-mediated phosphorylation. Upon T cell receptor engagement, PAG becomes dephosphorylated, thereby displacing Csk from the membrane and enabling the activation of the Src kinases Fyn and Lck. PAG negatively regulates TCR (T cell antigen receptor)-mediated signaling in T cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. It promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. It inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins and may be involved in cell adhesion signaling.
UniProt Q9JM80
Pathways p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events

Application Details

Product Details anti-PAG1 Antibody Target Details PAG1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.02 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at +4°C for short term storage, or at -20°C for long term storage. Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months