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anti-Rat (Rattus) SERPINE1 antibody for Immunocytochemistry

Recommended SERPINE1 Antibody (supplied by: Log in to see )

Antigen
serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies
  • PAI
  • PAI-1
  • PAI1
  • PLANH1
  • si:ch211-138a11.1
  • SERPINE1
  • Planh1
  • B230326M24Rik
  • PI-7
  • PI7
  • PN-1
  • Spi4
  • HsT1201
  • PAI-2
  • PAI2
  • PLANH2
  • PAI1A
  • Pai1
  • Pai1aa
  • Planh
  • RATPAI1A
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • plasminogen activator inhibitor 1
  • plasminogen activator inhibitor type 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 2
  • serpin peptidase inhibitor, clade B (ovalbumin), member 2
  • serpine1
  • SERPINE1
  • CpipJ_CPIJ011718
  • CpipJ_CPIJ016298
  • PAI-1
  • Serpine1
  • Serpine2
  • SERPINB2
Epitope
AA 300-400, Internal Region
40
15
13
13
12
11
7
5
5
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
337
113
107
32
11
9
3
2
2
1
1
1
1
Host
Rabbit
238
116
54
28
2
1
Clonality
Polyclonal
Conjugate
This SERPINE1 antibody is un-conjugated
44
28
17
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
300
208
102
65
43
42
23
23
18
18
17
16
9
6
6
6
5
5
4
4
4
1
1
1
1
1
Supplier
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Catalog No. ABIN446967
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1863010 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 24-402 Log in to see Polyclonal
1 ABIN4352947 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352927 ICC IF IHC IHC (fro) IHC (p) WB Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352948 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352935 ICC IF IHC (fro) IHC (p) WB Biotin Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352941 ICC IF IHC (fro) IHC (p) WB DyLight 650 Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352942 ICC IF IHC (fro) IHC (p) WB DyLight 680 Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352939 ICC IF IHC (fro) IHC (p) WB DyLight 488 Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352943 ICC IF IHC (fro) IHC (p) WB DyLight 755 Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352946 ICC IF IHC IHC (fro) IHC (p) WB PerCP Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352940 ICC IF IHC (fro) IHC (p) WB DyLight 550 Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352936 ICC IF IHC (fro) IHC (p) WB DyLight 350 Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352937 ICC IF IHC (fro) IHC (p) WB DyLight 405 Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352934 ICC IF IHC IHC (fro) IHC (p) WB Allophycocyanin Rabbit AA 300-400, Internal Region Log in to see Polyclonal
1 ABIN4352945 ICC IF IHC IHC (fro) IHC (p) WB PE Rabbit AA 300-400, Internal Region Log in to see Polyclonal

Top referenced anti-SERPINE1 antibody for Immunocytochemistry

Similar anti-SERPINE1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Antibody (Inhibition) 3 Antibodies 3 Antibodies
ELISA 30 Antibodies 24 Antibodies
ELISA (Capture) 3 Antibodies 3 Antibodies
ELISA (Detection) 14 Antibodies 12 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Functional Studies (Func) 4 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 72 Antibodies

General

Antigen serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies
Epitope AA 300-400, Internal Region
(40), (15), (13), (13), (12), (11), (7), (5), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(337), (113), (107), (32), (11), (9), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(238), (116), (54), (28), (2), (1)
Clonality
Polyclonal
Conjugate This SERPINE1 antibody is un-conjugated
(44), (28), (17), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(300), (208), (102), (65), (43), (42), (23), (23), (18), (18), (17), (16), (9), (6), (6), (6), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-SERPINE1 Antibody

Target Details SERPINE1 Application Details Handling References for anti-SERPINE1 Antibody (ABIN446967) Images
Specificity PAI-1 (N315) pAb detects endogenous levels of PAI-1 protein.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 300-400) [UniProt P05121]

Target Details SERPINE1

Product Details anti-SERPINE1 Antibody Application Details Handling References for anti-SERPINE1 Antibody (ABIN446967) Images back to top
Antigen
Alternative Name Serpin E1/PAI-1 (SERPINE1 Antibody Abstract)
Background Gene Symbol: SERPINE1
Molecular Weight Theoretical MW: 45 kDa
Gene ID 5054
UniProt P05121
Pathways p53 Signaling

Application Details

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Handling References for anti-SERPINE1 Antibody (ABIN446967) Images back to top
Application Notes Western Blot 1.0 μg/mL, Immunohistochemistry 1:50 - 1:100, Immunocytochemistry/Immunofluorescence 1:50 - 1:100, Immunohistochemistry-Paraffin 1:50 - 1:100, Immunohistochemistry-Frozen 1:50 - 1:100The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol specific for PAI1/Serpine1 antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry Protocol specific for PAI1/Serpine1 antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details References for anti-SERPINE1 Antibody (ABIN446967) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-SERPINE1 Antibody (ABIN446967)

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling Images back to top
Product cited in:

Sitaram, Mallikarjuna, Landström, Ljungberg: "Transforming growth factor-β promotes aggressiveness and invasion of clear cell renal cell carcinoma." in: Oncotarget, Vol. 7, Issue 24, pp. 35917-35931, 2016 (Sample species: Human). Further details: Western Blotting

Liao, Wang, Guo, Lin, Chang, Juo: "Let-7g improves multiple endothelial functions through targeting transforming growth factor-beta and SIRT-1 signaling." in: Journal of the American College of Cardiology, Vol. 63, Issue 16, pp. 1685-94, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Longchamp, Alonso, Dubuis, Allagnat, Berard, Meda, Saucy, Corpataux, Déglise, Haefliger: "The use of external mesh reinforcement to reduce intimal hyperplasia and preserve the structure of human saphenous veins." in: Biomaterials, Vol. 35, Issue 9, pp. 2588-99, 2014 (Sample species: Human). Further details: Western Blotting

Lee, Oh, Park, Na, Gil, Yang, Lee, Hong: "Urokinase, urokinase receptor, and plasminogen activator inhibitor-1 expression on podocytes in immunoglobulin A glomerulonephritis." in: The Korean journal of internal medicine, Vol. 29, Issue 2, pp. 176-82, 2014 (Sample species: Human).

Berard, Déglise, Alonso, Saucy, Meda, Bordenave, Corpataux, Haefliger: "Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins." in: Journal of vascular surgery, Vol. 57, Issue 5, pp. 1371-82, 2013 (Sample species: Human). Further details: Western Blotting

Dubuis, May, Alonso, Luca, Mylonaki, Meda, Delie, Jordan, Déglise, Corpataux, Saucy, Haefliger: "Atorvastatin-loaded hydrogel affects the smooth muscle cells of human veins." in: The Journal of pharmacology and experimental therapeutics, Vol. 347, Issue 3, pp. 574-81, 2013 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Cao, Szabolcs, Dutta, Yaqoob, Jagavelu, Wang, Leof, Urrutia, Shah, Mukhopadhyay: "Neuropilin-1 mediates divergent R-Smad signaling and the myofibroblast phenotype." in: The Journal of biological chemistry, Vol. 285, Issue 41, pp. 31840-8, 2010 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling References for anti-SERPINE1 Antibody (ABIN446967) back to top
Supplier Images
Western Blotting (WB) image for anti-SERPINE1 antibody (serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1) (AA 300-400) (ABIN446967) Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Extracts from Jurkat cells.
Immunofluorescence (IF) image for anti-SERPINE1 antibody (serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1) (AA 300-400) (ABIN446967) Immunocytochemistry/Immunofluorescence: Serpin E1/PAI-1 Antibody [NBP1-19773] - PAI1/...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SERPINE1 antibody (serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1) (AA 300-400) (ABIN446967) Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP1-19773] - Staining of PA...