anti-Dog (Canine) AKT2 antibody for Enzyme Immunoassay

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • AKT2
  • akt2
  • Akt2
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
451
167
126
37
33
27
26
25
22
22
21
21
21
4
3
2
2
1
1
Host
Rabbit
319
96
22
13
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
21
21
18
18
17
15
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
379
239
161
90
60
52
31
26
18
13
8
8
7
4
3
3
2
2
1
Supplier
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Catalog No. ABIN5551333
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5555628 EIA WB Goat C-Term Log in to see Polyclonal

Similar anti-AKT2 Antibodies

Application / Reactivity Human Rat (Rattus) Monkey Chicken Dog (Canine) Mouse (Murine) Cow (Bovine) Guinea Pig Hamster Pig (Porcine) Rabbit Sheep (Ovine) Xenopus laevis
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 3 Antibodies
Western Blotting (WB) 375 Antibodies 144 Antibodies 110 Antibodies 27 Antibodies 33 Antibodies 27 Antibodies 23 Antibodies 22 Antibodies 37 Antibodies 26 Antibodies 22 Antibodies 22 Antibodies 23 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Reactivity Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
(451), (167), (126), (37), (33), (27), (26), (25), (22), (22), (21), (21), (21), (4), (3), (2), (2), (1), (1)
Host Rabbit
(319), (96), (22), (13), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is un-conjugated
(21), (21), (18), (18), (17), (15), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(379), (239), (161), (90), (60), (52), (31), (26), (18), (13), (8), (8), (7), (4), (3), (3), (2), (2), (1)
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling Images
Specificity This antibody detects a ~65 kDa protein, corresponding to the Molecular Mass of Akt2 PKB beta on SDS-PAGE immunoblots. Does not react with PKB-alpha (Akt1) or PKB-gamma (Akt3).
Purification Affinity Chromatography
Immunogen A five residue synthetic peptide based on the Human Akt2, coupled to KLH
Isotype IgG

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT2 / PKB beta (AKT2 Antibody Abstract)
Background Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
Gene ID 208
NCBI Accession NP_001617
UniProt P31751
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
Application Notes Western blot (3): 1: 13000 dilution of SPC-148 was ufficient for detection of PKBβ in 20 μg ofHeLa cell lysate by ECL immunoblot analysis. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS, 0.09 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.