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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Dog (Canine)||Chicken||Guinea Pig||Hamster||Monkey||Pig (Porcine)||Rabbit||Sheep (Ovine)||Xenopus laevis|
|Western Blotting (WB)||328 Antibodies||105 Antibodies||86 Antibodies||23 Antibodies||28 Antibodies||23 Antibodies||19 Antibodies||19 Antibodies||28 Antibodies||21 Antibodies||19 Antibodies||19 Antibodies||21 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Luminex Assay (LMNX)||4 Antibodies|
|Immunoprecipitation (IP)||22 Antibodies||3 Antibodies|
|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis Alternatives|
|Conjugate||This AKT2 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-AKT2 AntibodyTarget Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN361723) Images
|Specificity||Detects ~65 kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).|
|Immunogen||A five residue synthetic peptide based on the human Akt2, coupled to KLH|
Target Details AKT2Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 Antibody (ABIN361723) Images back to top
|Alternative Name||AKT2 (AKT2 Antibody Abstract)|
|Background||Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).|
|Research Area||Cell Signaling, Phosphorylation|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling|
Application DetailsProduct Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 Antibody (ABIN361723) Images back to top
Recommended Dilution: WB (1:3000)
Optimal dilutions for assays should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 Antibody (ABIN361723) Images back to top
|Buffer||TBS, 50 % glycerol, 0.09 % sodium azide|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
References for anti-AKT2 Antibody (ABIN361723)Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002 (PubMed).
Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001 (PubMed).
Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998 (PubMed).
ImagesProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN361723) back to top