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anti-Human AKT2 antibody for Enzyme Immunoassay

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • 2410016A19Rik
  • AKT2
  • akt2-a
  • AW554154
  • cb945
  • HIHGHH
  • PKB
  • PKBB
  • pkbb
  • PKBBETA
  • PKBbeta
  • pkbbeta
  • pkbss
  • prkbb
  • PRKBB
  • RAC-BETA
  • rac-beta
  • v-akt2
Epitope
pSer474
54
43
41
30
25
16
11
11
10
7
6
5
5
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
404
128
105
30
28
25
23
21
21
19
19
19
19
2
1
1
Host
Rabbit
305
82
12
11
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
345
223
150
84
49
34
29
22
19
11
10
4
4
3
1
1
1
Supplier
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Catalog No. ABIN358304
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN359079 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
0.0008867729 ABIN359081 EIA IHC (p) WB Rabbit Ig Ser474 Log in to see Polyclonal 4
0.0008867729 ABIN359080 EIA WB Rabbit Ig Middle Region Log in to see Polyclonal 2
0.0008867729 ABIN359078 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 5
0.0008867729 ABIN264873 EIA WB Rabbit IgG Log in to see Polyclonal 3
0.0008867729 ABIN126856 EIA IHC (fro) WB Mouse IgG2a Log in to see 8B7
0.0008867729 ABIN1105276 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1B6 1
0.0008867729 ABIN950330 EIA WB Goat C-Term Log in to see Polyclonal
0.0008867729 ABIN614384 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1B6
0.0008867729 ABIN2715854 EIA ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-AKT2 antibody for Enzyme Immunoassay

Similar anti-AKT2 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 19 Antibodies
ELISA 214 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 34 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 29 Antibodies
Immunofluorescence (IF) 84 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 142 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 23 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 336 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Epitope pSer474
(54), (43), (41), (30), (25), (16), (11), (11), (10), (7), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(404), (128), (105), (30), (28), (25), (23), (21), (21), (19), (19), (19), (19), (2), (1), (1)
Host Rabbit
(305), (82), (12), (11), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA)
(345), (223), (150), (84), (49), (34), (29), (22), (19), (11), (10), (4), (4), (3), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN358304) Images
Specificity This antibody detects AKT2 / PKB beta pSer474.
Purification Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser474 of human AKT2.
Isotype Ig

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 Antibody (ABIN358304) Images back to top
Antigen
Alternative Name AKT2 / PKB beta (AKT2 Antibody Abstract)
Background AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
Molecular Weight 55769 Da
Gene ID 208, 9606
UniProt P31751
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 Antibody (ABIN358304) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 Antibody (ABIN358304) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-AKT2 Antibody (ABIN358304)

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Background publications

George, Rochford, Wolfrum, Gray, Schinner, Wilson, Soos, Murgatroyd, Williams, Acerini, Dunger, Barford, Umpleby, Wareham, Davies, Schafer, Stoffel, ORahilly, Barroso: "A family with severe insulin resistance and diabetes due to a mutation in AKT2." in: Science (New York, N.Y.), Vol. 304, Issue 5675, pp. 1325-8, 2004

Shim, Kang, Jeon, Kang, Chang, Kim: "Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8." in: Archives of biochemistry and biophysics, Vol. 425, Issue 2, pp. 214-20, 2004

Xu, Sakon, Nagano, Hiraoka, Yamamoto, Hayashi, Dono, Nakamori, Umeshita, Ito, Matsuura, Monden: "Akt2 expression correlates with prognosis of human hepatocellular carcinoma." in: Oncology reports, Vol. 11, Issue 1, pp. 25-32, 2003

Li, Leu, Cheong, Zhang, Baibakov, Shih, Birnbaum, Donowitz: "Akt2, phosphatidylinositol 3-kinase, and PTEN are in lipid rafts of intestinal cells: role in absorption and differentiation." in: Gastroenterology, Vol. 126, Issue 1, pp. 122-35, 2003

Vojtek, Taylor, DeRuiter, Yu, Figueroa, Kwok, Turner: "Akt regulates basic helix-loop-helix transcription factor-coactivator complex formation and activity during neuronal differentiation." in: Molecular and cellular biology, Vol. 23, Issue 13, pp. 4417-27, 2003

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN358304) back to top
Supplier Images
 image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (pSer474) (ABIN358304) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (pSer474) antibody