anti-Human AKT2 antibody for Flow Cytometry

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • AKT2
  • akt2
  • Akt2
Epitope
AA 2-481
66
43
33
29
19
15
15
12
11
9
7
6
6
6
5
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
468
170
129
38
34
28
27
26
23
23
22
22
22
4
3
2
2
1
1
Host
Mouse
327
105
22
13
3
2
Clonality
Monoclonal
Conjugate
This AKT2 antibody is un-conjugated
21
21
18
18
17
15
4
4
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC)
388
241
166
92
62
59
32
27
18
13
11
8
7
4
3
3
2
2
1
1
Supplier
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Catalog No. ABIN4899446
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.996978 ABIN652284 FACS IF WB Rabbit Ig Log in to see Polyclonal 2
13.996978 ABIN2452737 FACS WB Mouse IgG2a Log in to see 1A2
13.996978 ABIN2452738 FACS IP WB Mouse IgG1 Log in to see 8D9
13.996978 ABIN3029477 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 416-444 Log in to see Polyclonal
13.996978 ABIN4279058 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4H7
13.996978 ABIN3029470 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 93-123 Log in to see Polyclonal
13.996978 ABIN3029472 FACS IF ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN391022 FACS IF IHC (p) WB Rabbit Ig AA 416-444, C-Term Log in to see Polyclonal 1
1 ABIN391021 FACS IF IHC (p) WB Rabbit Ig AA 93-123, N-Term Log in to see Polyclonal 1
1 ABIN391023 FACS IF IHC (p) WB Rabbit Ig AA 93-123, Center Log in to see Polyclonal 2
1 ABIN1496565 FACS IP WB Mouse IgG1 Log in to see 8D9
1 ABIN2452740 FACS IF IHC WB Mouse IgG1 Log in to see 4H7
1 ABIN1496564 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4H7
1 ABIN5596673 ELISA FACS FM IHC WB Biotin Rat IgG2a Internal Region Log in to see 11F6-B2
1 ABIN5596672 ELISA FACS FM IHC WB Biotin Rat IgG2a Internal Region Log in to see 16G11-E8
1 ABIN5596675 ELISA FACS FM IHC WB FITC Rat IgG2a Internal Region Log in to see 11F6-B2
1 ABIN5596674 ELISA FACS FM IHC WB FITC Rat IgG2a Internal Region Log in to see 16G11-E8
1 ABIN4279059 ELISA FACS IHC WB Rat IgG2a kappa Log in to see 16G11-A7
1 ABIN5596670 ELISA FACS FM IHC WB APC Rat IgG2a Internal Region Log in to see 11F6-B2
1 ABIN5596677 ELISA FACS FM IHC WB PE Rat IgG2a Internal Region Log in to see 11F6-B2

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Epitope AA 2-481
(66), (43), (33), (29), (19), (15), (15), (12), (11), (9), (7), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(468), (170), (129), (38), (34), (28), (27), (26), (23), (23), (22), (22), (22), (4), (3), (2), (2), (1), (1)
Host Mouse
(327), (105), (22), (13), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT2 antibody is un-conjugated
(21), (21), (18), (18), (17), (15), (4), (4), (4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC)
(388), (241), (166), (92), (62), (59), (32), (27), (18), (13), (11), (8), (7), (4), (3), (3), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling References for anti-AKT2 antibody (ABIN4899446) Images
Specificity Detects human Akt2. Using direct ELISA, this antibody does not detect recombinant human (rh) Akt1 or rhAkt3.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt2, Asn2-Glu481, Accession # P31751
Clone 302501
Isotype IgG1

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 antibody (ABIN4899446) Images back to top
Antigen
Alternative Name Akt2 (AKT2 Antibody Abstract)
Background Alternative Names: EC 2.7.11, EC 2.7.11.1, Murine thymoma viral (v-akt) homolog-2, PKB beta, PKBB, PKBBETA, PRKBB, Protein kinase Akt-2, Protein kinase B beta, rac protein kinase beta, RAC-BETA, RAC-beta serine/threonine-protein kinase, RAC-PK-beta, v-akt murine thymoma viral oncogene homolog 2
Gene ID 208
UniProt P31751
Research Area Signaling, Cardiovascular, Atherosclerosis
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 antibody (ABIN4899446) Images back to top
Application Notes Immunohistochemistry 8-25 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 antibody (ABIN4899446) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-AKT2 antibody (ABIN4899446)

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Product cited in:

Hagemann, Bozanovic, Hooper, Ljubic, Slettenaar, Wilson, Singh, Gayther, Shepherd, Van Trappen: "Molecular profiling of cervical cancer progression." in: British journal of cancer, Vol. 96, Issue 2, pp. 321-8, 2007 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 antibody (ABIN4899446) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 2-481) antibody (ABIN4899446) Detection of Akt2 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast canc...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 2-481) antibody (ABIN4899446) Akt2 in Human Pancreatic Cancer Tissue. Akt2 was detected in immersion fixed paraffin...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 2-481) antibody (ABIN4899446) Akt2 in Human Pancreatic Cancer Tissue. Akt2 was detected in immersion fixed paraffin...
Flow Cytometry (FACS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 2-481) antibody (ABIN4899446) Detection of Akt2 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast canc...