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anti-Human AKT2 antibody for Flow Cytometry

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • AKT2
  • akt2
  • Akt2
Reactivity
Human, Mouse (Murine)
416
167
128
38
34
29
27
25
24
23
22
22
22
2
2
2
2
1
1
1
Host
Rabbit
306
86
12
11
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
351
222
151
84
52
31
31
27
18
13
11
7
4
3
3
2
2
1
Options
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Catalog No. ABIN652284
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.770219 ABIN4899446 FACS IHC Mouse IgG1 AA 2-481 Log in to see 302501 1
12.770219 ABIN2452738 FACS IP WB Mouse IgG1 Log in to see 8D9
12.770219 ABIN2452737 FACS WB Mouse IgG2a Log in to see 1A2
12.770219 ABIN3029477 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 416-444 Log in to see Polyclonal
12.770219 ABIN4279058 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4H7
12.770219 ABIN3029470 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 93-123 Log in to see Polyclonal
12.770219 ABIN3029472 FACS IF ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN391022 FACS IF IHC (p) WB Rabbit Ig AA 416-444, C-Term Log in to see Polyclonal 1
1 ABIN391023 FACS IF IHC (p) WB Rabbit Ig AA 93-123, Center Log in to see Polyclonal 2
1 ABIN391021 FACS IF IHC (p) WB Rabbit Ig AA 93-123, N-Term Log in to see Polyclonal 1
1 ABIN1496565 FACS IP WB Mouse IgG1 Log in to see 8D9
1 ABIN2452740 FACS IF IHC WB Mouse IgG1 Log in to see 4H7
1 ABIN1496564 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4H7
1 ABIN4279059 ELISA FACS IHC WB Rat IgG2a kappa Log in to see 16G11-A7
1 ABIN4279063 FACS Alexa Fluor 750 Mouse IgG1 AA 2-481 Log in to see 302501
1 ABIN4279062 FACS Alexa Fluor 647 Mouse IgG1 AA 2-481 Log in to see 302501
1 ABIN4279065 FACS Alexa Fluor 350 Mouse IgG1 AA 2-481 Log in to see 302501
1 ABIN4279066 FACS Alexa Fluor 405 Mouse IgG1 AA 2-481 Log in to see 302501
1 ABIN4279060 FACS Alexa Fluor 488 Mouse IgG1 AA 2-481 Log in to see 302501
1 ABIN4279061 FACS Alexa Fluor 700 Mouse IgG1 AA 2-481 Log in to see 302501

Top referenced anti-AKT2 antibody for Flow Cytometry

Similar anti-AKT2 Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 2 Antibodies 2 Antibodies
ELISA 62 Antibodies 220 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 11 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 24 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 70 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 144 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Reactivity Human, Mouse (Murine)
(416), (167), (128), (38), (34), (29), (27), (25), (24), (23), (22), (22), (22), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(306), (86), (12), (11), (3), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(351), (222), (151), (84), (52), (31), (31), (27), (18), (13), (11), (7), (4), (3), (3), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN652284) Images
Specificity This AKT2 antibody is generated from rabbits immunized with human AKT2 recombinant protein.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This AKT2 antibody is generated from rabbits immunized with human AKT2 recombinant protein.
Clone RB22732
Isotype Ig

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 Antibody (ABIN652284) Images back to top
Antigen
Alternative Name AKT2 (AKT2 Antibody Abstract)
Background AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Synonyms: RAC-beta serine/threonine protein kinase, RAC-PK-beta, Protein kinase Akt-2, Protein kinase B, beta, PKB beta
Molecular Weight 55768 DA
Gene ID 208
UniProt P31751
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 Antibody (ABIN652284) Images back to top
Application Notes WB = 1:1000, FACS = 1:10-50, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 Antibody (ABIN652284) Images back to top
Format Liquid
Concentration 0.37 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-AKT2 Antibody (ABIN652284)

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Background publications

Jones, Ganeshaguru, Anderson, Jackson, Bruckdorfer, Low, Palmqvist, Prentice, Hoffbrand, Mehta, Wickremasinghe: "Albumin activates the AKT signaling pathway and protects B-chronic lymphocytic leukemia cells from chlorambucil- and radiation-induced apoptosis." in: Blood, Vol. 101, Issue 8, pp. 3174-80, 2003

Brozinick, Roberts, Dohm: "Defective signaling through Akt-2 and -3 but not Akt-1 in insulin-resistant human skeletal muscle: potential role in insulin resistance." in: Diabetes, Vol. 52, Issue 4, pp. 935-41, 2003

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN652284) back to top
Supplier Images
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (ABIN652284) Western blot analysis of AKT2 Antibody (ABIN652284) in MDA-MB435 cell line lysates (3...
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (ABIN652284) Western blot analysis of AKT2 Antibody (ABIN652284) in mouse brain tissue lysates (35...
Flow Cytometry (FACS) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (ABIN652284) AKT2 Antibody (ABIN652284) flow cytometric analysis of NCI-H460 cells (right histogra...