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anti-Human AKT2 antibody for Western Blotting

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • AKT2
  • akt2
  • Akt2
Epitope
Middle Region
55
40
31
22
20
15
12
12
9
9
7
6
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
416
167
127
38
33
28
26
24
23
23
22
22
22
2
2
2
2
1
1
Host
Rabbit
306
86
12
11
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
351
222
151
85
52
32
31
27
18
13
11
7
4
3
3
2
2
1
Options
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Catalog No. ABIN2779323
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN391024 IF IHC (p) WB Rabbit Ig AA 452-481, Ser474 Log in to see Polyclonal 1
3.0726016 ABIN391022 FACS IF IHC (p) WB Rabbit Ig AA 416-444, C-Term Log in to see Polyclonal 1
3.0726016 ABIN359079 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
3.0726016 ABIN359080 EIA WB Rabbit Ig Middle Region Log in to see Polyclonal 2
3.0726016 ABIN391023 FACS IF IHC (p) WB Rabbit Ig AA 93-123, Center Log in to see Polyclonal 2
3.0726016 ABIN391021 FACS IF IHC (p) WB Rabbit Ig AA 93-123, N-Term Log in to see Polyclonal 1
3.0726016 ABIN4620529 EIA IHC (p) WB Rabbit Ig Ser474 Log in to see Polyclonal 4
3.0726016 ABIN2792145 WB Rabbit N-Term Log in to see Polyclonal 1
3.0726016 ABIN652284 FACS IF WB Rabbit Ig Log in to see Polyclonal 2
3.0726016 ABIN659024 IF IHC (p) WB Mouse IgG1 Log in to see 148CT5-4-1-4 3
3.0726016 ABIN4620528 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 5
3.0726016 ABIN1043775 IHC ELISA WB Rat IgG2a kappa Internal Region Log in to see 16G11-A7 1
3.0726016 ABIN1043774 IHC ELISA WB Rat IgG2a kappa Internal Region Log in to see 16G11-A7 1
3.0726016 ABIN393457 IF WB Mouse IgG1 kappa AA 100-189 Log in to see 1B3 5
3.0726016 ABIN393501 IF WB Mouse IgG1 kappa AA 100-189 Log in to see 1D9 5
3.0726016 ABIN393514 IF WB Mouse IgG1 kappa AA 100-189 Log in to see 1F3 5
3.0726016 ABIN1724720 ICC IHC ELISA WB Mouse IgG2b Log in to see 1B6 2
3.0726016 ABIN117900 WB Rabbit Log in to see Polyclonal 6
3.0726016 ABIN4279053 IHC IHC (p) WB Rabbit pSer474 Log in to see Polyclonal 1
3.0726016 ABIN657827 IHC (p) WB Rabbit Ig AA 93-122, N-Term Log in to see Polyclonal

Top referenced anti-AKT2 antibody for Western Blotting

Similar anti-AKT2 Antibodies

Application / Reactivity Human Rat (Rattus) Chicken Dog (Canine) Mouse (Murine) Cow (Bovine) Pig (Porcine) Xenopus laevis Zebrafish (Danio rerio)
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies 3 Antibodies
Western Blotting (WB) 347 Antibodies 144 Antibodies 111 Antibodies 27 Antibodies 33 Antibodies 28 Antibodies 25 Antibodies 24 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Immunoprecipitation (IP) 27 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Epitope Middle Region
(55), (40), (31), (22), (20), (15), (12), (12), (9), (9), (7), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(416), (167), (127), (38), (33), (28), (26), (24), (23), (23), (22), (22), (22), (2), (2), (2), (2), (1), (1)
Host Rabbit
(306), (86), (12), (11), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(351), (222), (151), (85), (52), (32), (31), (27), (18), (13), (11), (7), (4), (3), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN2779323) Images
Blocking Peptide Blocking peptide for this product available: ABIN973084
Sequence TSEVDTRYFD DEFTAQSITI TPPDRYDSLG LLELDQRTHF PQFSYSASIR
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against AKT2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human AKT2

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 Antibody (ABIN2779323) Images back to top
Antigen
Alternative Name AKT2 (AKT2 Antibody Abstract)
Background AKT2 belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene encoding AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Molecular Weight 56 kDa
Gene ID 208
NCBI Accession NM_001626, NP_001617
UniProt P31751
Research Area Cardiovascular, Atherosclerosis, Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 Antibody (ABIN2779323) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 481 AA

Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 Antibody (ABIN2779323) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-AKT2 Antibody (ABIN2779323)

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Product cited in:

Tan, Timakhov, Rao, Altomare, Xu, Liu, Gao, Jhanwar, Di Cristofano, Wiest, Knepper, Testa: "A novel recurrent chromosomal inversion implicates the homeobox gene Dlx5 in T-cell lymphomas from Lck-Akt2 transgenic mice." in: Cancer research, Vol. 68, Issue 5, pp. 1296-302, 2008

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN2779323) back to top
Supplier Images
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (Middle Region) (ABIN2779323) WB Suggested Anti-AKT2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (Middle Region) (ABIN2779323) Host: Rabbit
Target Name: AKT2
Sample Tissue: Human Fetal Muscle
...