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anti-Mouse (Murine) AKT2 antibody for Immunohistochemistry

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • 2410016A19Rik
  • AKT2
  • akt2-a
  • AW554154
  • cb945
  • HIHGHH
  • PKB
  • PKBB
  • pkbb
  • PKBBETA
  • PKBbeta
  • pkbbeta
  • pkbss
  • prkbb
  • PRKBB
  • RAC-BETA
  • rac-beta
  • v-akt2
Epitope
pSer474
54
43
41
30
25
16
11
11
10
7
6
5
5
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
402
126
103
30
28
25
23
21
21
19
19
19
19
2
1
1
Host
Rabbit
304
81
12
11
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
342
222
148
83
48
34
28
22
19
11
11
4
4
3
1
1
1
Supplier
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Catalog No. ABIN1531768
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.2292046 ABIN1532797 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.2292046 ABIN2704466 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.2292046 ABIN1869977 IHC WB Rabbit IgG pSer474 Log in to see Polyclonal
0.2292046 ABIN2162549 IHC WB Rabbit IgG Ser474 Log in to see Polyclonal
0.2292046 ABIN2159040 FACS IF IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.2292046 ABIN2162548 IHC ELISA WB Rabbit IgG Ser474 Log in to see Polyclonal
0.2292046 ABIN2469563 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.2292046 ABIN2159036 IF IHC WB Mouse IgG1 Log in to see 148CT5-4-1-4
0.2292046 ABIN447009 ICC IF IHC (p) IHC WB Rabbit Thr468 Log in to see Polyclonal
0.2292046 ABIN2159039 IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.2292046 ABIN2432447 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0011344266 ABIN362104 IHC WB Rabbit IgG pSer474 Log in to see Polyclonal 3
0.0011344266 ABIN1892282 IHC ELISA WB PE Rabbit IgG N-Term, AA 92-122 Log in to see Polyclonal
0.0011344266 ABIN1892279 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 92-122 Log in to see Polyclonal
0.0011344266 ABIN1892278 IHC ELISA WB APC Rabbit IgG N-Term, AA 92-122 Log in to see Polyclonal
0.0011344266 ABIN1892281 IHC ELISA WB FITC Rabbit IgG N-Term, AA 92-122 Log in to see Polyclonal
0.0011344266 ABIN1892280 IHC ELISA WB Biotin Rabbit IgG N-Term, AA 92-122 Log in to see Polyclonal
0.0011344266 ABIN1870912 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011344266 ABIN1842376 IHC WB Rabbit IgG pSer474 Log in to see Polyclonal 2
0.0011344266 ABIN1892283 IHC ELISA WB HRP Rabbit IgG N-Term, AA 92-122 Log in to see Polyclonal

Top referenced anti-AKT2 antibody for Immunohistochemistry

Similar anti-AKT2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 39 Antibodies 42 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 3 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 22 Antibodies 20 Antibodies
Immunofluorescence (IF) 33 Antibodies 32 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 91 Antibodies
Flow Cytometry (FACS) 6 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Epitope pSer474
(54), (43), (41), (30), (25), (16), (11), (11), (10), (7), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(402), (126), (103), (30), (28), (25), (23), (21), (21), (19), (19), (19), (19), (2), (1), (1)
Host Rabbit
(304), (81), (12), (11), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(342), (222), (148), (83), (48), (34), (28), (22), (19), (11), (11), (4), (4), (3), (1), (1), (1)
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling Images
Specificity Akt2 (Phospho-Ser474) Antibody detects endogenous levels of Akt2 only when phosphorylated at Ser474.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Akt2 around the phosphorylation site of Ser474.
Isotype IgG

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Akt2 (AKT2 Antibody Abstract)
Background Molecular weight of target protein: 55 kDa
Synonyms: kinase Akt2, PKB beta, Protein kinase Akt-2, Protein kinase B, beta, RAC-beta serine/threonine protein kinase, RAC-PK-beta, v-akt murine thymoma viral oncogene homolog 2
Molecular Weight 55 kDa
Gene ID 208
OMIM 164731
UniProt P31751
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.631535 (NCBI Gene Symbol: AKT2)

Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (pSer474) (ABIN1531768) Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30', using...
Immunohistochemistry (IHC) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (pSer474) (ABIN1531768) Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Akt2 (...