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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||39 Antibodies||42 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||22 Antibodies||20 Antibodies|
|Immunofluorescence (IF)||33 Antibodies||32 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||47 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Western Blotting (WB)||91 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies|
|Epitope||AA 454-481, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AKT2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-AKT2 AntibodyTarget Details AKT2 Application Details Handling Images
|Sequence||DRYDSLGLLE LDQRTHFPQF SYSASIRE|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for RAC-beta serine/threonine-protein kinase(AKT2) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: v-akt murine thymoma viral oncogene homolog 2
Protein Name: RAC-beta serine/threonine-protein kinase
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human AKT2(454-481aa DRYDSLGLLELDQRTHFPQFSYSASIRE), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.|
Target Details AKT2Product Details anti-AKT2 Antibody Application Details Handling Images back to top
|Alternative Name||AKT2 (AKT2 Antibody Abstract)|
|Background||AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. This gene is mapped to 19q13.2. AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.|
|Research Area||Cardiovascular, Atherosclerosis, Signaling|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling|
Application DetailsProduct Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for AKT2 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling back to top