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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)|
|Immunofluorescence (fixed cells) (IF/ICC)||7 Antibodies||2 Antibodies||3 Antibodies|
|Western Blotting (WB)||352 Antibodies||146 Antibodies||111 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Luminex Assay (LMNX)||4 Antibodies|
|Immunoprecipitation (IP)||27 Antibodies||9 Antibodies||10 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||55 Antibodies||33 Antibodies||25 Antibodies|
|Immunohistochemistry (IHC)||151 Antibodies||72 Antibodies||55 Antibodies|
|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AKT2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-AKT2 AntibodyTarget Details AKT2 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Akt2 protein only when phosphorylated at Ser474. This antibody does not cross-react with Akt1 and Akt3 protein when phosphorylated at the corresponding residues.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details AKT2Product Details anti-AKT2 Antibody Application Details Handling Images back to top
|Alternative Name||AKT2 / PKB beta (AKT2 Antibody Abstract)|
|Background||AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase|
|Molecular Weight||approx. 60 kDa|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C|
Application DetailsProduct Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling back to top