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anti-Rat (Rattus) AKT2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • 2410016A19Rik
  • AKT2
  • akt2-a
  • AW554154
  • cb945
  • HIHGHH
  • PKB
  • PKBB
  • pkbb
  • PKBBETA
  • PKBbeta
  • pkbbeta
  • pkbss
  • prkbb
  • PRKBB
  • RAC-BETA
  • rac-beta
  • v-akt2
Epitope
pSer474
54
43
41
30
25
16
11
11
10
7
6
5
5
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
404
127
104
30
28
25
23
21
21
19
19
19
19
2
1
1
Host
Rabbit
305
82
12
11
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
344
223
150
84
48
34
29
22
19
11
11
4
4
3
1
1
1
Supplier
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Catalog No. ABIN498710
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN498587 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN3043781 IHC (fro) IHC (p) WB Rabbit IgG AA 454-481, C-Term Log in to see Polyclonal
0.0008982679 ABIN302641 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN1105276 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1B6 1
0.0008982679 ABIN614384 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1B6
0.0008982679 ABIN447009 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN677080 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN677087 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN677078 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN4279053 IHC IHC (p) WB Rabbit pSer474 Log in to see Polyclonal
0.0008982679 ABIN1731656 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN4279056 ELISA IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN4279057 ELISA IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN3029473 IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN4279055 ELISA ICC IF IHC (p) WB Mouse IgG2b Log in to see 1B6

Top referenced anti-AKT2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-AKT2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 336 Antibodies 109 Antibodies 91 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Immunoprecipitation (IP) 23 Antibodies 3 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies 22 Antibodies 16 Antibodies
Immunohistochemistry (IHC) 142 Antibodies 59 Antibodies 47 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 2 Antibodies 2 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Epitope pSer474
(54), (43), (41), (30), (25), (16), (11), (11), (10), (7), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(404), (127), (104), (30), (28), (25), (23), (21), (21), (19), (19), (19), (19), (2), (1), (1)
Host Rabbit
(305), (82), (12), (11), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(344), (223), (150), (84), (48), (34), (29), (22), (19), (11), (11), (4), (4), (3), (1), (1), (1)
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling Images
Specificity This antibody detects endogenous levels of Akt2 protein only when phosphorylated at Ser474. This antibody does not cross-react with Akt1 and Akt3 protein when phosphorylated at the corresponding residues.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT2 / PKB beta (AKT2 Antibody Abstract)
Background AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
Molecular Weight approx. 60 kDa
Gene ID 208
NCBI Accession NP_001617
UniProt P31751
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (pSer474) (ABIN498710) Immunohistochemistry (IHC) analyzes of p-Akt2 antibody in paraffin-embedded hu...
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (pSer474) (ABIN498710) Western blot (WB) analysis of p-AKT2 antibody at 1/500 dilution