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anti-Human CHUK antibody for Immunofluorescence

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase
  • chuk
  • CHUK
  • Chuk
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
355
133
87
19
4
4
1
Host
Mouse
276
82
12
1
Clonality
Monoclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
8
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
311
154
96
66
63
51
41
28
26
18
9
5
2
2
1
1
1
1
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Catalog No. ABIN252647
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.710068 ABIN499968 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
11.710068 ABIN499966 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
11.710068 ABIN499970 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
11.710068 ABIN4324237 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 2
11.710068 ABIN4324239 CyTOF FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231 43
11.710068 ABIN1107728 EIA FACS IF WB Mouse IgG1 Log in to see 3G12H9 1
11.710068 ABIN1871859 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal
11.710068 ABIN2490846 IF Rabbit IgG Tyr199 Log in to see Polyclonal
11.710068 ABIN2490847 IF Rabbit IgG pTyr199 Log in to see Polyclonal
11.710068 ABIN4324236 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
11.710068 ABIN4324233 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 3G12H9
11.710068 ABIN4324238 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
11.710068 ABIN560377 IF PLA ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 1F8
11.710068 ABIN560376 IF ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 4D11
11.710068 ABIN560378 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 4B8
11.710068 ABIN560379 IF IHC (p) PLA ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 2G4
11.710068 ABIN2405010 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
11.710068 ABIN931520 IF ELISA WB Mouse IgG1 Log in to see 3G12H9
1 ABIN393762 IF WB Mouse IgG1 kappa AA 646-745 Log in to see 4B8 5
1 ABIN1497517 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 3G12H9

Top referenced anti-CHUK antibody for Immunofluorescence

Similar anti-CHUK Antibodies

Application / Reactivity Rat (Rattus) Primate
Chromatin Immunoprecipitation (ChIP) 2 Antibodies 2 Antibodies
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 18 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 16 Antibodies
Immunofluorescence (IF) 5 Antibodies 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies 18 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 53 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human, Mouse (Murine), Primate, Rat (Rattus)
(355), (133), (87), (19), (4), (4), (1)
Host Mouse
(276), (82), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(311), (154), (96), (66), (63), (51), (41), (28), (26), (18), (9), (5), (2), (2), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN252647) Images
Specificity An 85 kDa band should be observed.
Purification Protein G purified
Immunogen This antibody was raised against a His-tagged full-length human IKK alpha protein.
Clone 14A231
Isotype IgG1 kappa

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK Antibody (ABIN252647) Images back to top
Antigen
Alternative Name IKK alpha (CHUK Antibody Abstract)
Background Gene Symbol: CHUK
Gene ID 1147
UniProt O15111
Research Area Immunology, Innate Immunity, Atherosclerosis, Transcription Factors, Apoptosis/Necrosis
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK Antibody (ABIN252647) Images back to top
Application Notes Western Blot 1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry (Intracellular): 0.25-0.5 μg/10^6 cells, Immunocytochemistry/Immunofluorescence 1:10, Immunoprecipitation 1-2 μg/mL, Immunohistochemistry-Paraffin 5 μg/mL, Immunohistochemistry-Frozen, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP)Use in Flow Intracellular reported in scientific literature (PMID 24804954) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25133425). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in chromatin immunoprecipitation reported in scientific literature (PMID 27705798).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK Antibody (ABIN252647) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS - BSA free
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CHUK Antibody (ABIN252647)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Product cited in:

Vincendeau, Hadian, Messias, Brenke, Halander, Griesbach, Greczmiel, Bertossi, Stehle, Nagel, Demski, Velvarska, Niessing, Geerlof, Sattler, Krappmann: "Inhibition of Canonical NF-κB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction." in: Scientific reports, Vol. 6, pp. 18934, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Shintaku, Peterson, Talbert, Gu, Ladner, Williams, Mousavi, Wang, Sartorelli, Guttridge: "MyoD Regulates Skeletal Muscle Oxidative Metabolism Cooperatively with Alternative NF-κB." in: Cell reports, Vol. 17, Issue 2, pp. 514-526, 2016 Method employed by authors: Western Blotting (WB)

Alameda, Gaspar, Ramírez, Navarro, Page, Suárez-Cabrera, Fernández, Mérida, Paramio, García-Fernández, Fernández-Aceñero, Casanova: "Deciphering the role of nuclear and cytoplasmic IKKα in skin cancer." in: Oncotarget, Vol. 7, Issue 20, pp. 29531-47, 2016 Method employed by authors: Immunocytochemistry (ICC), Immunofluorescence (IF) (Sample species: Human).

Mihalas, Meffert: "IKK kinase assay for assessment of canonical NF-κB activation in neurons." in: Methods in molecular biology (Clifton, N.J.), Vol. 1280, pp. 61-74, 2015

Remouchamps, Dejardin: "Methods to assess the activation of the alternative (noncanonical) NF-κB pathway by non-death TNF receptors." in: Methods in molecular biology (Clifton, N.J.), Vol. 1280, pp. 103-19, 2015

Ren, Takahashi, Liu, Loughran, Sun, Wang, Cheng: "HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains." in: Oncogene, Vol. 34, Issue 3, pp. 334-45, 2015 (Sample species: Human). Further details: Western Blotting

Gray, McCorkell, Chunduru, McKinlay, May: "Negative feedback regulation of NF-κB-inducing kinase is proteasome-dependent but does not require cellular inhibitors of apoptosis." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 341-6, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Richardson, Hammond, Coulombe, Saloranta, Nousiainen, Salonen, Berry, Hanley, Headon, Karikoski, Dixon: "Periderm prevents pathological epithelial adhesions during embryogenesis." in: The Journal of clinical investigation, Vol. 124, Issue 9, pp. 3891-900, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Gray, Remouchamps, McCorkell, Solt, Dejardin, Orange, May: "Noncanonical NF-κB signaling is limited by classical NF-κB activity." in: Science signaling, Vol. 7, Issue 311, pp. ra13, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Pradhan, Pratheek, Garai, Kumar, Meena, Ghosh, Singh, Kumari, Chandrashekar, Goswami, Chattopadhyay, Kar, Maiti: "Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells." in: Cell biology international, Vol. 38, Issue 10, pp. 1118-31, 2014 (Sample species: Human). Further details: Flow Cytometry,Immunochromatography

Das, Manna, Sinha, Datta, Das, Chakraborty, Ghosh, Saha, Dey: "Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model." in: PLoS ONE, Vol. 9, Issue 5, pp. e97599, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Buhrmann, Busch, Shayan, Shakibaei: "Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells." in: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22048-62, 2014 (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Schwitalla, Fingerle, Cammareri, Nebelsiek, Göktuna, Ziegler, Canli, Heijmans, Huels, Moreaux, Rupec, Gerhard, Schmid, Barker, Clevers, Lang, Neumann, Kirchner, Taketo, van den Brink, Sansom, Arkan et al.: "Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties. ..." in: Cell, Vol. 152, Issue 1-2, pp. 25-38, 2013 Further details: Western Blotting

Mihalas, Araki, Huganir, Meffert: "Opposing action of nuclear factor ?B and Polo-like kinases determines a homeostatic end point for excitatory synaptic adaptation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 42, pp. 16490-501, 2013 Further details: Immunoprecipitation

Bimonte, Barbieri, Palma, Luciano, Rea, Arra: "Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer." in: BioMed research international, Vol. 2013, pp. 810423, 2013 (Sample species: Human). Further details: Western Blotting

Cui, Li, Zhu, Liu, Songyang, Wang, Wang: "NLRP4 negatively regulates type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4." in: Nature immunology, Vol. 13, Issue 4, pp. 387-95, 2012

Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012

Bakkar, Ladner, Canan, Liyanarachchi, Bal, Pant, Periasamy, Li, Janssen, Guttridge: "IKK? and alternative NF-?B regulate PGC-1? to promote oxidative muscle metabolism." in: The Journal of cell biology, Vol. 196, Issue 4, pp. 497-511, 2012

Phromnoi, Reuter, Sung, Prasad, Kannappan, Yadav, Chanmahasathien, Limtrakul, Aggarwal et al.: "A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IκB kinase activation and sensitizes tumor cells to apoptosis by ..." in: Molecular pharmacology, Vol. 79, Issue 2, pp. 279-89, 2011 (Sample species: Human). Further details: Western Blotting

Hao, Rizzo, Osipo, Pannuti, Wyatt, Cheung, Sonenshein, Osborne, Miele: "Notch-1 activates estrogen receptor-alpha-dependent transcription via IKKalpha in breast cancer cells." in: Oncogene, Vol. 29, Issue 2, pp. 201-13, 2010

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN252647) back to top
Supplier Images
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody
Simple Western (SimWes) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Simple Western: IKK alpha Antibody (14A231) [ABIN2526474] - Simple Western lane view ...
Flow Cytometry (FACS) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Flow Cytometry: IKK alpha Antibody (14A231) [ABIN2526474] - Analysis using Azide Free...
Flow Cytometry (FACS) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Flow Cytometry: IKK alpha Antibody (14A231) [ABIN2526474] - Intracellular staining of...
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Western Blot: IKK alpha Antibody (14A231) [ABIN2526474] - Total protein from various ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) [ABIN2526474] - Analysis u...
Immunofluorescence (IF) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) [ABIN2526474] - A...
Immunofluorescence (IF) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) [ABIN2526474] - A...