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anti-Human CHUK antibody for Immunofluorescence

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • AI256658
  • CHUK
  • Chuk1
  • Fbx24
  • Fbxo24
  • IKBKA
  • ikk
  • IKK-alpha
  • IKK1
  • ikk1
  • Ikka
  • IKKA
  • ikka
  • ikkalpha
  • NFKBIKA
  • TCF16
  • zgc:56539
Epitope
C-Term
62
29
23
17
15
15
13
13
12
11
10
9
9
9
8
7
7
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
339
129
84
19
4
4
Host
Rabbit
270
73
11
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
10
9
8
8
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
303
145
95
64
55
46
37
26
22
9
4
4
2
2
1
1
Supplier
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Catalog No. ABIN499966
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3221521 ABIN499968 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.3221521 ABIN499970 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.3221521 ABIN1107728 EIA FACS IF WB Mouse IgG1 Log in to see 3G12H9 1
0.3221521 ABIN2490846 IF Rabbit IgG Tyr199 Log in to see Polyclonal
0.3221521 ABIN2490847 IF Rabbit IgG pTyr199 Log in to see Polyclonal
0.3221521 ABIN560376 IF ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 4D11
0.3221521 ABIN560378 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 4B8
0.3221521 ABIN560379 IF IHC (p) PLA ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 2G4
0.3221521 ABIN560377 IF PLA ELISA WB Mouse IgG1 kappa AA 646-745, partial Log in to see 1F8
0.3221521 ABIN931520 IF ELISA WB Mouse IgG1 Log in to see 3G12H9
0.3221521 ABIN2405010 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010820389 ABIN393762 IF WB Mouse IgG1 kappa AA 646-745 Log in to see 4B8 5
0.0010820389 ABIN1497517 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 3G12H9
0.0010820389 ABIN189444 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y463
0.0010820389 ABIN393623 IF WB Mouse IgG1 kappa AA 646-745 Log in to see 2G4
0.0010820389 ABIN598611 IF ELISA WB Mouse IgG1, kappa Log in to see 4B8
0.0010820389 ABIN598612 IF ELISA WB Mouse IgG1, kappa Log in to see 2G4
0.0010820389 ABIN303080 EIA IF IHC (p) IP WB Rabbit Log in to see Polyclonal
0.0010820389 ABIN931518 IF ELISA WB Mouse IgG1 Log in to see 3G12
0.0010820389 ABIN218505 IF IHC (p) IP ELISA WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-CHUK antibody for Immunofluorescence

Similar anti-CHUK Antibodies

Application / Reactivity Human
Control Peptide (CP) 1 Antibodies
ELISA 131 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 27 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 38 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 85 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 66 Antibodies
Immunoprecipitation (IP) 57 Antibodies
Proximity Ligation Assay (PLA) 5 Antibodies
Western Blotting (WB) 292 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope C-Term
(62), (29), (23), (17), (15), (15), (13), (13), (12), (11), (10), (9), (9), (9), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(339), (129), (84), (19), (4), (4)
Host Rabbit
(270), (73), (11), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(15), (11), (10), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(303), (145), (95), (64), (55), (46), (37), (26), (22), (9), (4), (4), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN499966) Images
Specificity IKK alpha antibody was raised against a peptide corresponding to amino acids 716 to 734 of human IKK alpha, which differs from corresponding murine sequence by four amino acids. This antibody has no cross response to IKKb or IKKg.
Purification Affinity chromatography purified via peptide column
Immunogen Human IKK alpha (C-Terminus 1) Peptide
Isotype IgG

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK Antibody (ABIN499966) Images back to top
Antigen
Alternative Name IKKA (CHUK Antibody Abstract)
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The long-sought IkappaB kinase (IKK), which phosphorylates IkappaB, and mediates IΚB degradation and NF-kappaB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKa and IKKbeta interact with each other and both are essential for the NF-kappaB activation. IKKalpha specifically phosphorylates IkB-alpha. IKKalpha is expressed in variety of human tissues.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16
Gene ID 1147
NCBI Accession NP_001269
UniProt O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK Antibody (ABIN499966) Images back to top
Application Notes ELISA. Western Blot: 1: 1000 to 1: 2000 dilution. An 85 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK Antibody (ABIN499966) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-CHUK Antibody (ABIN499966)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Background publications

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997

Zandi, Rothwarf, Delhase, Hayakawa, Karin: "The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation." in: Cell, Vol. 91, Issue 2, pp. 243-52, 1997

Woronicz, Gao, Cao, Rothe, Goeddel: "IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 866-9, 1997

Régnier, Song, Gao, Goeddel, Cao, Rothe: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN499966) back to top
Supplier Images
Immunofluorescence (IF) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (C-Term) (ABIN499966) Immunocytochemistry of IKK alpha in Jurkat cells with AP30403PU-N IKK alpha antibody ...
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (C-Term) (ABIN499966) Western blot analysis of IKK alpha in HeLa whole cell lysate with AP30403PU-N IKK alp...