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anti-Human CHUK antibody for Western Blotting

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase
  • chuk
  • CHUK
  • Chuk
Epitope
pSer176, pSer177
49
28
17
15
15
15
11
10
10
9
9
8
8
8
7
7
7
6
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
338
150
103
20
12
11
10
7
6
5
5
4
2
1
Host
Rabbit
258
82
12
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
8
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
305
147
87
70
59
47
43
29
26
19
9
5
3
3
2
2
1
1
1
Options
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Catalog No. ABIN1531285
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN393762 IF WB Mouse IgG1 kappa AA 646-745 Log in to see 4B8 5
3.0726016 ABIN252647 ChIP FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231 69
3.0726016 ABIN499968 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
3.0726016 ABIN499966 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
3.0726016 ABIN499970 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
3.0726016 ABIN1881204 WB Rabbit AA 553-582, C-Term Log in to see Polyclonal 5
3.0726016 ABIN1881203 WB Rabbit AA 84-112, N-Term Log in to see Polyclonal 5
3.0726016 ABIN967550 IP WB Mouse IgG2b, kappa Log in to see B78-1 5
3.0726016 ABIN969056 ICC FACS ELISA WB Mouse IgG1 Log in to see 3G12H9 1
3.0726016 ABIN969054 ELISA WB Mouse IgG1 AA 500-590 Log in to see 7C10B6 2
3.0726016 ABIN969055 ELISA WB Mouse IgG1 Log in to see 3G12 1
3.0726016 ABIN4324237 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 2
3.0726016 ABIN222974 IP WB Rabbit IgG Log in to see Polyclonal 2
3.0726016 ABIN4324239 CyTOF FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231 43
3.0726016 ABIN4324257 ELISA WB Goat C-Term Log in to see Polyclonal 1
3.0726016 ABIN1531873 IHC ELISA WB Rabbit IgG pThr23 Log in to see Polyclonal
3.0726016 ABIN1107727 EIA WB Mouse IgG1 Log in to see 3G12 1
3.0726016 ABIN1107728 EIA FACS IF WB Mouse IgG1 Log in to see 3G12H9 1
3.0726016 ABIN3043812 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal
3.0726016 ABIN3043147 WB Rabbit IgG AA 728-745, C-Term Log in to see Polyclonal

Top referenced anti-CHUK antibody for Western Blotting

Similar anti-CHUK Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 2 Antibodies 2 Antibodies
ELISA 18 Antibodies 32 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 19 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 69 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope pSer176, pSer177
(49), (28), (17), (15), (15), (15), (11), (10), (10), (9), (9), (8), (8), (8), (7), (7), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(338), (150), (103), (20), (12), (11), (10), (7), (6), (5), (5), (4), (2), (1)
Host Rabbit
(258), (82), (12), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(305), (147), (87), (70), (59), (47), (43), (29), (26), (19), (9), (5), (3), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN1531285) Images
Specificity IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody detects endogenous levels of IKK-alpha only when phosphorylated at
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Ser177.
Isotype IgG

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK Antibody (ABIN1531285) Images back to top
Antigen
Alternative Name IKK-alpha (CHUK Antibody Abstract)
Background Molecular weight of target protein: 84 kDa
Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IkappaB kinase, IkBKA, IKK-A, IKK-alpha, IKK1, IKKA, Inhibitor of nuclear factor kappa-B kinase alpha subunit, kinase IKK-alpha, NFKBIKA, Nuclear factor NFkappaB inhibitor kinase alpha, I-kappa-B kinase 2, I-kappa-B-kinase beta, IkBKB, IKK-B, IKK- beta, IKK2, IKKB, kinase IKK-beta, NFKBIKB, Nuclear factor NF-kappa- B inhibitor kinase beta
Molecular Weight 84 kDa
Gene ID 1147, 3551
OMIM 600664
UniProt O14920, O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK Antibody (ABIN1531285) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.198998, Hs.656458 (NCBI Gene Symbol: CHUK)

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK Antibody (ABIN1531285) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-CHUK Antibody (ABIN1531285)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Background publications

Régnier, Song, Gao, Goeddel, Cao, Rothe: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN1531285) back to top
Supplier Images
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (pSer176) (ABIN1531285) Western blot analysis of extracts from NIH-3T3 cells treated with TNF 20ng/ml 30', us...
Immunohistochemistry (IHC) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (pSer176) (ABIN1531285) Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha (Phos...