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anti-Human CHUK antibody for Western Blotting

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • AI256658
  • CHUK
  • Chuk1
  • Fbx24
  • Fbxo24
  • IKBKA
  • ikk
  • IKK-alpha
  • IKK1
  • ikk1
  • Ikka
  • IKKA
  • ikka
  • ikkalpha
  • NFKBIKA
  • TCF16
  • zgc:56539
Epitope
AA 646-745
62
29
23
17
15
15
13
13
12
11
10
9
9
9
7
7
7
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
311
109
83
4
4
Host
Mouse
266
50
10
1
Clonality
Monoclonal
Conjugate
This CHUK antibody is un-conjugated
14
10
10
8
8
8
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
276
138
90
40
39
36
30
22
10
6
4
2
2
1
1
Supplier
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Catalog No. ABIN393762
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.125708 ABIN1531285 IHC ELISA WB Rabbit IgG pSer176, pSer177 Log in to see Polyclonal 3
0.125708 ABIN499966 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.125708 ABIN499968 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.125708 ABIN499970 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.125708 ABIN969055 ELISA WB Mouse IgG1 Log in to see 3G12 1
0.125708 ABIN969056 ICC FACS ELISA WB Mouse IgG1 Log in to see 3G12H9 1
0.125708 ABIN969054 ELISA WB Mouse IgG1 AA 500-590 Log in to see 7C10B6 2
0.125708 ABIN967550 IP WB Mouse IgG2b, kappa Log in to see B78-1 5
0.125708 ABIN1881204 WB Rabbit AA 553-582, C-Term Log in to see Polyclonal 5
0.125708 ABIN1881203 WB Rabbit AA 84-112, N-Term Log in to see Polyclonal 5
0.125708 ABIN222974 IP WB Rabbit IgG Log in to see Polyclonal 2
0.125708 ABIN1531873 IHC ELISA WB Rabbit IgG pThr23 Log in to see Polyclonal
0.125708 ABIN1107727 EIA WB Mouse IgG1 Log in to see 3G12 1
0.125708 ABIN1107728 EIA FACS IF WB Mouse IgG1 Log in to see 3G12H9 1
0.125708 ABIN1532904 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.125708 ABIN2707590 IHC WB Rabbit N-Term Log in to see Polyclonal
0.125708 ABIN2707589 IHC WB Rabbit N-Term, pSer23 Log in to see Polyclonal
0.125708 ABIN3043812 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal
0.125708 ABIN3043147 WB Rabbit IgG AA 728-745, C-Term Log in to see Polyclonal
0.125708 ABIN498205 IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-CHUK antibody for Western Blotting

Similar anti-CHUK Antibodies

Application / Reactivity Human
Control Peptide (CP) 1 Antibodies
ELISA 124 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 41 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 38 Antibodies
Proximity Ligation Assay (PLA) 5 Antibodies
Western Blotting (WB) 265 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope AA 646-745
(62), (29), (23), (17), (15), (15), (13), (13), (12), (11), (10), (9), (9), (9), (7), (7), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(311), (109), (83), (4), (4)
Host Mouse
(266), (50), (10), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHUK antibody is un-conjugated
(14), (10), (10), (8), (8), (8), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (IF), Western Blotting (WB)
(276), (138), (90), (40), (39), (36), (30), (22), (10), (6), (4), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN393762) Images
Specificity CHUK (NP_001269, 646 a.a. ~ 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen CHUK (NP_001269, 646 a.a. ~ 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4B8
Isotype IgG1 kappa

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK Antibody (ABIN393762) Images back to top
Antigen
Alternative Name CHUK (CHUK Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 84654 DA
Gene ID 12406
NCBI Accession NP_001269
Research Area Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK Antibody (ABIN393762) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK Antibody (ABIN393762) Images back to top
Concentration 0.37mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-CHUK Antibody (ABIN393762)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Background publications

Li, Morgan, Choksi, Zhang, Kim, Liu: "MicroRNAs modulate the noncanonical transcription factor NF-kappaB pathway by regulating expression of the kinase IKKalpha during macrophage differentiation." in: Nature immunology, Vol. 11, Issue 9, pp. 799-805, 2010

Rotman, Koh, Zmuda, Kleiner, Liang: "The association of genetic variability in patatin-like phospholipase domain-containing protein 3 (PNPLA3) with histological severity of nonalcoholic fatty liver disease." in: Hepatology (Baltimore, Md.), Vol. 52, Issue 3, pp. 894-903, 2010

Jugessur, Shi, Gjessing, Lie, Wilcox, Weinberg, Christensen, Boyles, Daack-Hirsch, Nguyen, Christiansen, Lidral, Murray: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Olson, Wang, Pankratz, Fredericksen, Vachon, Vierkant, Cerhan, Couch: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN393762) back to top
Supplier Images
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (AA 646-745) (ABIN393762) Immunofluorescence of monoclonal antibody to CHUK on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (AA 646-745) (ABIN393762) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibody (Image 2)