anti-Mouse (Murine) CHUK antibody for ELISA

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase
  • chuk
  • CHUK
  • Chuk
Epitope
pSer176, pSer177
51
32
17
17
15
15
11
10
10
9
9
9
8
8
7
7
7
7
6
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
353
153
104
20
12
11
10
7
6
5
5
4
2
1
Host
Rabbit
273
83
12
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
8
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
318
150
94
78
62
48
44
29
26
19
9
5
3
3
2
2
1
1
1
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Catalog No. ABIN1531285
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1531873 IHC ELISA WB Rabbit IgG pThr23 Log in to see Polyclonal
1 ABIN1532904 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN3182039 ELISA IHC WB Rabbit IgG pThr23 Log in to see Polyclonal
1 ABIN3185147 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal
1 ABIN364294 IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2888444 IHC (p) ELISA WB Rabbit IgG pThr23 Log in to see Polyclonal
1 ABIN601601 IHC ELISA WB Rabbit IgG pSer180, pSer181 Log in to see Polyclonal
1 ABIN601603 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN220382 ICC IP ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN218505 IF IHC (p) IP ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1733082 IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2885478 IHC ELISA WB Rabbit IgG pSer176 Log in to see Polyclonal
1 ABIN2467456 IP IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1964353 IHC ELISA WB APC Rabbit IgG AA 551-578 Log in to see Polyclonal
1 ABIN1961974 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 551-578 Log in to see Polyclonal
1 ABIN1975370 IHC ELISA WB PE Rabbit IgG AA 551-578 Log in to see Polyclonal
1 ABIN1972730 IHC ELISA WB HRP Rabbit IgG AA 551-578 Log in to see Polyclonal
1 ABIN1970789 IHC ELISA WB FITC Rabbit IgG AA 551-578 Log in to see Polyclonal
1 ABIN1966952 IHC ELISA WB Biotin Rabbit IgG AA 551-578 Log in to see Polyclonal
1 ABIN2619263 ELISA IHC WB Rabbit IgG AA 550-578 Log in to see Polyclonal

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope pSer176, pSer177
(51), (32), (17), (17), (15), (15), (11), (10), (10), (9), (9), (9), (8), (8), (7), (7), (7), (7), (6), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(353), (153), (104), (20), (12), (11), (10), (7), (6), (5), (5), (4), (2), (1)
Host Rabbit
(273), (83), (12), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(318), (150), (94), (78), (62), (48), (44), (29), (26), (19), (9), (5), (3), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling ProductDetails: References for anti-CHUK antibody (ABIN1531285) Images
Specificity IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody detects endogenous levels of IKK-alpha only when phosphorylated at
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Ser177.
Isotype IgG

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling ProductDetails: References for anti-CHUK antibody (ABIN1531285) Images back to top
Antigen
Alternative Name IKK-alpha (CHUK Antibody Abstract)
Background Molecular weight of target protein: 84 kDa
Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IkappaB kinase, IkBKA, IKK-A, IKK-alpha, IKK1, IKKA, Inhibitor of nuclear factor kappa-B kinase alpha subunit, kinase IKK-alpha, NFKBIKA, Nuclear factor NFkappaB inhibitor kinase alpha, I-kappa-B kinase 2, I-kappa-B-kinase beta, IkBKB, IKK-B, IKK- beta, IKK2, IKKB, kinase IKK-beta, NFKBIKB, Nuclear factor NF-kappa- B inhibitor kinase beta
Molecular Weight 84 kDa
Gene ID 1147, 3551
OMIM 600664
UniProt O14920, O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling ProductDetails: References for anti-CHUK antibody (ABIN1531285) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.198998, Hs.656458 (NCBI Gene Symbol: CHUK)

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details ProductDetails: References for anti-CHUK antibody (ABIN1531285) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-CHUK antibody (ABIN1531285)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Background publications

Régnier, Song, Gao, Goeddel, Cao, Rothe: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling ProductDetails: References for anti-CHUK antibody (ABIN1531285) back to top
Supplier Images
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibody (ABIN1531285) Western blot analysis of extracts from NIH-3T3 cells treated with TNF 20ng/ml 30', us...
Immunohistochemistry (IHC) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibody (ABIN1531285) Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha (Phos...