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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||13 Antibodies||28 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies||3 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||20 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||8 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies||22 Antibodies|
|Immunohistochemistry (IHC)||38 Antibodies||60 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||24 Antibodies||46 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies||27 Antibodies|
|Western Blotting (WB)||55 Antibodies|
|Antigen||conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CHUK antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-CHUK AntibodyTarget Details CHUK Application Details Handling Images
|Immunogen||A synthetic peptide corresponding to residues in the N-term of human IKK-alpha was used as immunogen.|
Target Details CHUKProduct Details anti-CHUK Antibody Application Details Handling Images back to top
|Alternative Name||IKKA (CHUK Antibody Abstract)|
IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composedof IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on aserine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it fordegradation via ubiquination. Activation of NFKB by TNF requires phosphorylation ofIKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has beendemonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4).In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELAto be recruited to the NF-kappa-B-responsive promoters in order to mediate thecytokine-induced phosphorylation and subsequent acetylation of specific residues inhistone H3 (5). Alternate Names: anti-CHUK1 antibody, anti-Conserved Helix Loop Helix Ubiquitous Kinase antibody,anti-Conserved helix loop ubiquitous kinase antibody, anti-I Kappa B Kinase 1 antibody,anti-I Kappa B Kinase Alpha antibody, anti-IkappaB kinase antibody, anti-IkB kinasealpha subunit antibody, anti-IKBKA antibody, anti-IKK 1 antibody, anti-IKK A antibody,anti-IKK a kinase antibody, anti-IKK1 antibody, anti-IKKA antibody, anti-Inhibitor OfKappa Light Polypeptide Gene Enhancer In B Cells antibody, anti-Inhibitor Of NuclearFactor Kappa B Kinase Alpha Subunit antibody, anti-NFKBIKA antibody, anti-NuclearFactor Kappa B Inhibitor Kinase Alpha antibody, anti-Nuclear factor NF kappa B inhibitorkinase alpha antibody, anti-Nuclear factor NFkappaB inhibitor kinase alpha antibody,anti-Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody,anti-TCF16 antibody.
Gene Symbol: CHUK
|Gene ID||1147, 12675, 309361|
|Pathways||PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 85kDa.
Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol specific for IKK alpha Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-CHUK Antibody Target Details CHUK Application Details Handling back to top