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anti-Mouse (Murine) CHUK antibody for Flow Cytometry

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • AI256658
  • CHUK
  • Chuk1
  • Fbx24
  • Fbxo24
  • IKBKA
  • ikk
  • IKK-alpha
  • IKK1
  • ikk1
  • Ikka
  • IKKA
  • ikka
  • ikkalpha
  • NFKBIKA
  • TCF16
  • zgc:56539
Epitope
N-Term
63
29
23
17
15
15
13
13
12
11
10
9
9
9
8
7
7
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
340
129
84
19
4
4
Host
Rabbit
271
73
11
1
Clonality
Monoclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
10
9
8
8
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
304
145
95
64
55
47
37
26
22
10
4
4
2
2
1
1
Supplier
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Available images

Catalog No. ABIN189444
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010469276 ABIN4324231 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324248 FACS IHC (p) IP WB PE Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324249 FACS IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324250 FACS IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324251 FACS IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324252 FACS IHC (p) IP WB DyLight 405LS Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324253 FACS IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324254 FACS IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324255 FACS IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324256 FACS IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324240 FACS IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324241 FACS IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324242 FACS IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324243 FACS IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324244 FACS IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324246 FACS IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324247 FACS IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324239 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231
0.0010469276 ABIN4324245 FACS IHC (p) IP WB HRP Mouse IgG1 kappa Log in to see 14A231

Similar anti-CHUK Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 13 Antibodies 28 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies 20 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 5 Antibodies
Immunofluorescence (IF) 4 Antibodies 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 38 Antibodies 60 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies 46 Antibodies
Immunoprecipitation (IP) 6 Antibodies 27 Antibodies
Western Blotting (WB) 55 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope N-Term
(63), (29), (23), (17), (15), (15), (13), (13), (12), (11), (10), (9), (9), (9), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(340), (129), (84), (19), (4), (4)
Host Rabbit
(271), (73), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHUK antibody is un-conjugated
(15), (11), (10), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(304), (145), (95), (64), (55), (47), (37), (26), (22), (10), (4), (4), (2), (2), (1), (1)
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling Images
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in the N-term of human IKK-alpha was used as immunogen.
Clone Y463
Isotype IgG

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling Images back to top
Antigen
Alternative Name IKKA (CHUK Antibody Abstract)
Background IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composedof IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on aserine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it fordegradation via ubiquination. Activation of NFKB by TNF requires phosphorylation ofIKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has beendemonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4).In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELAto be recruited to the NF-kappa-B-responsive promoters in order to mediate thecytokine-induced phosphorylation and subsequent acetylation of specific residues inhistone H3 (5). Alternate Names: anti-CHUK1 antibody, anti-Conserved Helix Loop Helix Ubiquitous Kinase antibody,anti-Conserved helix loop ubiquitous kinase antibody, anti-I Kappa B Kinase 1 antibody,anti-I Kappa B Kinase Alpha antibody, anti-IkappaB kinase antibody, anti-IkB kinasealpha subunit antibody, anti-IKBKA antibody, anti-IKK 1 antibody, anti-IKK A antibody,anti-IKK a kinase antibody, anti-IKK1 antibody, anti-IKKA antibody, anti-Inhibitor OfKappa Light Polypeptide Gene Enhancer In B Cells antibody, anti-Inhibitor Of NuclearFactor Kappa B Kinase Alpha Subunit antibody, anti-NFKBIKA antibody, anti-NuclearFactor Kappa B Inhibitor Kinase Alpha antibody, anti-Nuclear factor NF kappa B inhibitorkinase alpha antibody, anti-Nuclear factor NFkappaB inhibitor kinase alpha antibody,anti-Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody,anti-TCF16 antibody.
Gene Symbol: CHUK
Gene ID 1147, 12675, 309361
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 85kDa.
Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for IKK alpha Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (N-Term) (ABIN189444) Western blot analysis on Daudi cell lysate using anti-IKK-alpha (N-term) RabMAb (cat....
Immunohistochemistry (IHC) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (N-Term) (ABIN189444) Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-...