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anti-Mouse (Murine) CHUK antibody for Immunoprecipitation

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • AI256658
  • CHUK
  • Chuk1
  • Fbx24
  • Fbxo24
  • IKBKA
  • ikk
  • IKK-alpha
  • IKK1
  • ikk1
  • Ikka
  • IKKA
  • ikka
  • ikkalpha
  • NFKBIKA
  • TCF16
  • zgc:56539
Epitope
N-Term
62
29
23
17
15
15
13
13
12
11
10
9
9
9
8
7
7
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
312
109
83
4
4
Host
Rabbit
266
51
10
1
Clonality
Monoclonal
Conjugate
This CHUK antibody is un-conjugated
14
10
10
8
8
8
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
277
138
90
40
40
36
30
22
10
6
4
2
2
1
1
Supplier
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Available images

Catalog No. ABIN189444
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN615031 EIA IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089235755 ABIN116874 EIA IP WB Rabbit C-Term Log in to see Polyclonal
0.00089235755 ABIN2467456 IP IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089235755 ABIN2470216 IF IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.00089235755 ABIN2405010 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089235755 ABIN1733082 IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089235755 ABIN2973335 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-CHUK Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 13 Antibodies 28 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 3 Antibodies
Immunofluorescence (IF) 4 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 37 Antibodies 59 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies 26 Antibodies
Immunoprecipitation (IP) 6 Antibodies 8 Antibodies
Western Blotting (WB) 54 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope N-Term
(62), (29), (23), (17), (15), (15), (13), (13), (12), (11), (10), (9), (9), (9), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(312), (109), (83), (4), (4)
Host Rabbit
(266), (51), (10), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHUK antibody is un-conjugated
(14), (10), (10), (8), (8), (8), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(277), (138), (90), (40), (40), (36), (30), (22), (10), (6), (4), (2), (2), (1), (1)
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling Images
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in the N-term of human IKK-alpha was used as immunogen.
Clone Y463
Isotype IgG

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling Images back to top
Antigen
Alternative Name IKKA (CHUK Antibody Abstract)
Background IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composedof IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on aserine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it fordegradation via ubiquination. Activation of NFKB by TNF requires phosphorylation ofIKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has beendemonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4).In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELAto be recruited to the NF-kappa-B-responsive promoters in order to mediate thecytokine-induced phosphorylation and subsequent acetylation of specific residues inhistone H3 (5). Alternate Names: anti-CHUK1 antibody, anti-Conserved Helix Loop Helix Ubiquitous Kinase antibody,anti-Conserved helix loop ubiquitous kinase antibody, anti-I Kappa B Kinase 1 antibody,anti-I Kappa B Kinase Alpha antibody, anti-IkappaB kinase antibody, anti-IkB kinasealpha subunit antibody, anti-IKBKA antibody, anti-IKK 1 antibody, anti-IKK A antibody,anti-IKK a kinase antibody, anti-IKK1 antibody, anti-IKKA antibody, anti-Inhibitor OfKappa Light Polypeptide Gene Enhancer In B Cells antibody, anti-Inhibitor Of NuclearFactor Kappa B Kinase Alpha Subunit antibody, anti-NFKBIKA antibody, anti-NuclearFactor Kappa B Inhibitor Kinase Alpha antibody, anti-Nuclear factor NF kappa B inhibitorkinase alpha antibody, anti-Nuclear factor NFkappaB inhibitor kinase alpha antibody,anti-Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody,anti-TCF16 antibody.
Gene Symbol: CHUK
Gene ID 1147, 12675, 309361
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 85kDa.
Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for IKK alpha Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (N-Term) (ABIN189444) Western blot analysis on Daudi cell lysate using anti-IKK-alpha (N-term) RabMAb (cat....
Immunohistochemistry (IHC) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (N-Term) (ABIN189444) Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-...