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anti-Human PPP2R4 antibody for Enzyme Immunoassay

Recommended PPP2R4 Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) Antibodies
  • 2610042B21Rik
  • 6330556D22Rik
  • C77440
  • N28142
  • PP2A
  • PR53
  • PR65
  • PTPA
  • PTPRA
Epitope
C-Term
9
8
5
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
55
26
23
3
3
2
1
1
1
1
1
Host
Goat
39
13
8
Clonality
Polyclonal
Conjugate
This PPP2R4 antibody is un-conjugated
2
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
54
20
19
12
8
8
5
3
2
1
1
1
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Catalog No. ABIN452430
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008871704 ABIN1108715 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 4D9 2

Top referenced anti-PPP2R4 antibody for Enzyme Immunoassay

Similar anti-PPP2R4 Antibodies

Application / Reactivity Human Cow (Bovine) Dog (Canine) Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 51 Antibodies 4 Antibodies 4 Antibodies 25 Antibodies 21 Antibodies
Immunoprecipitation (IP) 3 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies 2 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 18 Antibodies 7 Antibodies 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (IF) 12 Antibodies 3 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 3 Antibodies

General

Antigen Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) Antibodies
Epitope C-Term
(9), (8), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(55), (26), (23), (3), (3), (2), (1), (1), (1), (1), (1)
Host Goat
(39), (13), (8)
Clonality
Polyclonal
Conjugate This PPP2R4 antibody is un-conjugated
(2), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(54), (20), (19), (12), (8), (8), (5), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PPP2R4 Antibody

Target Details PPP2R4 Application Details Handling References for anti-PPP2R4 Antibody (ABIN452430) Images
Sequence c-GSLLPIHPVT SG
Specificity This antibody is expected to recognize all reported isoforms (NP_821068.1, NP_066954.2, NP_821070.1).
Cross-Reactivity (Details) Species reactivity (expected):Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse, Rat.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography.
Immunogen Peptide with sequence from the C-Terminus of the protein sequence according to NP_821068.1, NP_066954.2, NP_821070.1. Genename: PPP2R4

Target Details PPP2R4

Product Details anti-PPP2R4 Antibody Application Details Handling References for anti-PPP2R4 Antibody (ABIN452430) Images back to top
Antigen
Alternative Name PPP2R4 / PTPA (PPP2R4 Antibody Abstract)
Background Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.Synonyms: PP2A subunit B' PR53 isoform, Phosphotyrosyl phosphatase activator, Serine/threonine-protein phosphatase 2A regulatory subunit B'
Gene ID 5524
NCBI Accession NP_001180326
UniProt Q15257
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Handling References for anti-PPP2R4 Antibody (ABIN452430) Images back to top
Application Notes Peptide ELISA: 1/32000. Western Blot: Use at 0.5-2.0 μg/mL. Approx 37 kDa band observed in Human Liver lysates and in lysates of cell line HepG2(Calculated MW of 36.7 kDa according to NP_066954.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details References for anti-PPP2R4 Antibody (ABIN452430) Images back to top
Concentration 0.5 mg/mL
Buffer Tris-saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PPP2R4 Antibody (ABIN452430)

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details Handling Images back to top
Background publications

Gushiken, Patel, Liu, Pradhan, Bergeron, Peng, Vijayan: "Protein phosphatase 2A negatively regulates integrin alpha(IIb)beta(3) signaling." in: The Journal of biological chemistry, Vol. 283, Issue 19, pp. 12862-9, 2008

Images

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details Handling References for anti-PPP2R4 Antibody (ABIN452430) back to top
Supplier Images
 image for anti-PPP2R4 antibody (Protein Phosphatase 2A Activator, Regulatory Subunit 4) (C-Term) (ABIN452430) anti-Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) (C-Term) antibody