|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Dog (Canine)|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||1 Antibodies||2 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Western Blotting (WB)||60 Antibodies||6 Antibodies||5 Antibodies||33 Antibodies||29 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies||3 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies||4 Antibodies||4 Antibodies|
|Immunohistochemistry (IHC)||21 Antibodies||11 Antibodies||9 Antibodies|
|Immunofluorescence (IF)||14 Antibodies|
|Antigen||Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PPP2R4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-PPP2R4 AntibodyTarget Details PPP2R4 Application Details Handling References for anti-PPP2R4 Antibody (ABIN452430) Images
|Specificity||This antibody is expected to recognize all reported isoforms (NP_821068.1, NP_066954.2, NP_821070.1).|
Species reactivity (expected):Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse, Rat.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography.|
|Immunogen||Peptide with sequence from the C-Terminus of the protein sequence according to NP_821068.1, NP_066954.2, NP_821070.1. Genename: PPP2R4|
Target Details PPP2R4Product Details anti-PPP2R4 Antibody Application Details Handling References for anti-PPP2R4 Antibody (ABIN452430) Images back to top
|Alternative Name||PPP2R4 / PTPA (PPP2R4 Antibody Abstract)|
|Background||Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.Synonyms: PP2A subunit B' PR53 isoform, Phosphotyrosyl phosphatase activator, Serine/threonine-protein phosphatase 2A regulatory subunit B'|
|Pathways||PI3K-Akt Signaling, M Phase|
Application DetailsProduct Details anti-PPP2R4 Antibody Target Details PPP2R4 Handling References for anti-PPP2R4 Antibody (ABIN452430) Images back to top
Peptide ELISA: 1/32000. Western Blot: Use at 0.5-2.0 μg/mL. Approx 37 kDa band observed in Human Liver lysates and in lysates of cell line HepG2(Calculated MW of 36.7 kDa according to NP_066954.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details References for anti-PPP2R4 Antibody (ABIN452430) Images back to top
|Buffer||Tris-saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PPP2R4 Antibody (ABIN452430)Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details Handling Images back to top
Gushiken, Patel, Liu, Pradhan, Bergeron, Peng, Vijayan: "Protein phosphatase 2A negatively regulates integrin alpha(IIb)beta(3) signaling." in: The Journal of biological chemistry, Vol. 283, Issue 19, pp. 12862-9, 2008
ImagesProduct Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details Handling References for anti-PPP2R4 Antibody (ABIN452430) back to top