GPA33 Protein (AA 22-235) (His tag)
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- Target See all GPA33 Proteins
- GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-235
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This GPA33 protein is labelled with His tag.
- Purpose
- Recombinant Human Glycoprotein A33/GPA33/GPA33 (C-6His)
- Sequence
- ISVETPQDVL RASQGKSVTL PCTYHTSTSS REGLIQWDKL LLTHTERVVI WPFSNKNYIH GELYKNRVSI SNNAEQSDAS ITIDQLTMAD NGTYECSVSL MSDLEGNTKS RVRLLVLVPP SKPECGIEGE TIIGNNIQLT CQSKEGSPTP QYSWKRYNIL NQEQPLAQPA SGQPVSLKNI STDTSGYYIC TSSNEEGTQF CNITVAVRSP SMNVVDHHHH HH
- Characteristics
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Recombinant Human Glycoprotein A33/GPA33/GPA33 (C-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product GPA33 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))
- Alternative Name
- Glycoprotein A33/GPA33 (GPA33 Products)
- Synonyms
- A33 Protein, 2010310L10Rik Protein, 2210401D16Rik Protein, BB116197 Protein, glycoprotein A33 Protein, glycoprotein A33 (transmembrane) Protein, GPA33 Protein, Gpa33 Protein
- Background
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Recombinant Human Glycoprotein A33/GPA33 produced by transfected human cells is a secreted protein with sequence (Ile22-Val235) of Human GPA33 fused with a polyhistidine tag at the C-terminus.
Human Glycoprotein A33 (GPA33) is a single-pass type I membrane protein, belongs to the CTX family of cell adhesion molecular within the immunoglobulin family, can be expressed in normal gastrointestinal epithelium and in 95 % of colon cancers. GPA33 consists of one Ig-like C2-type domain and one Ig-like V-type domain. The predicted mature protein includes a single transmembrane domain, a extracellular region and a intracellular tail. Intracellular traffic and recycling to the cell surface appear to play an important role in GPA33 function and to have an influence on its surface density superseding translation regulation. GPA33 has become a promising target of immunologic therapy strategies. GPA33 may also play a important role in cell-cell recognition and signaling. - Molecular Weight
- 24.66 kDa
- UniProt
- Q99795
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