GM-CSF Protein

Catalog No. ABIN1305143

Product Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Brand: BD Pharmingen™

Specificity: Biological Activity: Activity Range is measured in a Proliferation Assay, using MC/9 indicator cells
Specific Activity: 0.25 - 5.0 x 10^9 Units/mg
ED50: 2 - 35 pg/ml
Observed dose response: 60 fold.
A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Characteristics: Recombinat mouse GM-CSF is supplied as a frozen liquid comprised of 0.22 um sterile-filtered aqueous buffered solution (ph 7.2) and containing 2 mg/ml biotchnology grade, low endotoxin bovine serum albumin, with no preservatives. The mouse GM-CSF was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law. Recombinant mouse GM-CSF is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution (pH 7.2) and containing 2 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.

Purification: Immunoaffinity Chromatography
Recombinat mouse GM-CSF is supplied as a frozen liquid comprised of 0.22 um sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives. Recombinant mouse GM-CSF is ≥ 95 % pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of mouse GM-CSF, as measured in chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant mouse GM-CSF from BD Biosciences Pharmingen is useful as a quantitative standard for measuring mouse GM-CSF protein levels in a GM-CSF specific sandwich ELISA with the purified MP1-22E9 antibody (Cat. No. 554404) as a capture antibody and the biotinylated MP1-31G6 antibody (Cat. No. 554407) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse GM-CSF standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Ligand Blocking Control for Immunofluorescent Staining and Flow Cytometric Analysis: The recombinant mouse GM-CSF can be used as a blocking control to demonstrate specificity of GM-CSF staining (for instance, by the PE-MP1-22E9 antibody, Cat. No. 554406). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

Comment:

BD Pharmingen™ Recombinant Mouse GM-CSF - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Preservative: Azide free

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. This product should not be diluted to less than 5 µg/ml for long term storage.
Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may affect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Thompson-Snipes, Dhar, Bond, Mosmann, Moore, Rennick: "Interleukin 10: a novel stimulatory factor for mast cells and their progenitors." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 507-10, (1991) (PubMed).

Gasson: "Molecular physiology of granulocyte-macrophage colony-stimulating factor." in: Blood, Vol. 77, Issue 6, pp. 1131-45, (1991) (PubMed).

Price, Mochizuki, March, Cosman, Deeley, Klinke, Clevenger, Gillis, Baker, Urdal: "Expression, purification and characterization of recombinant murine granulocyte-macrophage colony-stimulating factor and bovine interleukin-2 from yeast." in: Gene, Vol. 55, Issue 2-3, pp. 287-93, (1987) (PubMed).

Miyatake, Otsuka, Yokota, Lee, Arai: "Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2561-8, (1985) (PubMed).

Gough, Gough, Metcalf, Kelso, Grail, Nicola, Burgess, Dunn: "Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor." in: Nature, Vol. 309, Issue 5971, pp. 763-7, (1984) (PubMed).

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF (CSF2 Products)

Synonyms: GMCSF Protein, GM-CSF Protein, CSF2 Protein, Csfgm Protein, Gm-CSf Protein, MGI-IGM Protein, Gm-csf Protein, Gmcsf Protein, CSF Protein, colony stimulating factor 2 Protein, colony stimulating factor 2 (granulocyte-macrophage) Protein, CSF2 Protein, Csf2 Protein

Background: Mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine made by activated T cells, macrophages, vascular endothelial cells and fibroblasts. It has potent stimulatory effects on the growth and differentiation of bone marrow progenitor cells that generate granulocytes, monocytes/macrophages, and megakaryocytes. In peripheral tissues, GM-CSF can act on mature leukocytes to promote inflammatory responses. Mouse GM-CSF runs as a 15.5 - 19 kD band in reducing and non-reducing SDS-PAGE analysis. Mouse GM-CSF protein contains 124 amino acid residues.

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin