Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 8-144) Protein

Details for Product No. ABIN1019699, Supplier: Log in to see
Protein Name
  • GMCSF
  • GM-CSF
  • CSF2
  • Csfgm
  • Gm-CSf
  • MGI-IGM
  • Gm-csf
  • Gmcsf
  • CSF
  • colony stimulating factor 2
  • colony stimulating factor 2 (granulocyte-macrophage)
  • CSF2
  • Csf2
Protein Characteristics
AA 8-144
28
6
4
2
2
1
1
1
1
1
1
1
1
Origin
Human
58
27
14
5
5
4
2
2
1
1
1
1
1
Source
Tobacco (Nicotiana benthamiana)
68
13
9
6
6
3
1
1
1
1
1
1
Protein Type
Recombinant
Application
Cell Culture (CC), Western Blotting (WB)
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Sequence HHHHHHHHHH APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
Specificity Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific GM-CSF antibody.
Characteristics Molecular Formula: C699H1077N201O206S8
Isoelectric Point: 6,21
Extinction Coefficient: E 0.1 % (1g/L) = 0.898 (A 280 nm)
Endotoxin Level < 0.04 EU/µg protein (LAL method)
Protein Name
Background Synonyms: Granulocyte-macrophage colony-stimulating factor (GM-CSF), Colony-stimulating factor (CSF), Molgramostin, Sargramostim
GMCSF is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. It´s involved in differentiation of dendritic cells and is a key factor in differentiation pathways leading form stem cells. GMCSF is produced by several cell types as monocytes, fibroblasts, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hemopoietic environment and peripheral site of inflammation. Human GMCF is an important therapeutic cytokine used in the treatment of myeloid leukemia, neutropenia and aplastic anemia and it could become interesting in the treatment following bone marrow transplantation. It performs its biological activity by binding to a specific receptor complex which is composed of a cytokine-specific alpha chain and beta chain, shared with the receptors for interleukin-3 and interleukin-5. GMCSR has been identified to mediate the activation of Jak-Stat and MAPK pathways.
UniProt P04141
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
Comment

rHuman GM-CSF is a glycosylated polypeptide chain containing 127 amino acids (18-144 aa CSF2_HUMAN P04141 ), fused to 10 His tag at N-terminal. rHuman GM-CSF migrate as a broad band between 15 and 25 kDa due to post-translation modification, in particular glycosylation. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Granulocyte-macrophage colony-stimulating factor (GM-CSF) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal free product. The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with coomassie blue.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng /µL. Optimal concentration should be determined for specific application and cell lines.
Buffer 10 mM PBS buffer pH 7.6 and 0.2 M NaCl
Storage 4 °C
Supplier Images
 image for Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 8-144) protein (ABIN1019699) Analysis of recombinant GM-CSF with specific anti-human GM-CSF by Western Blot. MWM: ...
 image for Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 8-144) protein (ABIN1019699) SDS-PAGE analysis of recombinant GM-CSF. Samples were loaded in 15 % SDS-polyacrylami...
 image for Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 8-144) protein (ABIN1019699) The activity of recombinant human GM-CSF is determined by the dose-dependent inductio...
 image for Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 8-144) protein (ABIN1019699) Effect of rH GM-CSF on kerotinocytes migration (HaCaT cells). 24 hours effect on ski...
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