GM-CSF Protein

Catalog No. ABIN1305144

Product Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Brand: BD Pharmingen™

Specificity: Biological Activity Range: Measured in a proliferation assay using MC/9 indicator cells
Specific Activity: 0.1 - 1.0 X 10^9 Units /mg
ED50: 10 - 100 pg/ml
Observed dose response: 100 fold A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Characteristics: Recombinant rat GM-CSF is supplied as a frozen liquid comprised of 0.22 µm sterile filtered aqueous buffered solution and containing 1.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat GM-CSF is purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant rat GM-CSF is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing bovine serum albumin and glycerol, with no preservatives. Recombinant rat GM-CSF is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of rat GM-CSF, as measured in chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant rat GM-CSF is useful as a quantitative standard for measuring rat GM-CSF protein levels in a GM-CSF specific sandwich ELISA with the purified B61-5/B61-9 antibody cocktail as a capture reagent and the biotinylated monoclonal B61-10 antibody as the detection antibody.

Comment:

BD Pharmingen™ Recombinant Rat GM-CSF - Reactivity Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a cytokine which is made by activated T cells, macrophages, vascular endothelial cells and fibroblasts. It has potent stimulatory effects on the growth and differentiation of bone marrow progenitor cells that generate granulocytes monocytes/macrophages, and megakaryocytes. In peripheral tissues, GM-CSF can act on mature leukocytes to promote inflammatory responses.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Zhao, Xia, Fritz: "Interleukin-3 and encephalitogenic activity of SJL/J myelin basic protein-specific T cell lines." in: Journal of neuroimmunology, Vol. 43, Issue 1-2, pp. 69-78, (1993) (PubMed).

Gasson: "Molecular physiology of granulocyte-macrophage colony-stimulating factor." in: Blood, Vol. 77, Issue 6, pp. 1131-45, (1991) (PubMed).

Miyatake, Otsuka, Yokota, Lee, Arai: "Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2561-8, (1985) (PubMed).

Gough, Gough, Metcalf, Kelso, Grail, Nicola, Burgess, Dunn: "Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor." in: Nature, Vol. 309, Issue 5971, pp. 763-7, (1984) (PubMed).

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF (CSF2 Products)

Synonyms: GMCSF Protein, GM-CSF Protein, CSF2 Protein, Csfgm Protein, Gm-CSf Protein, MGI-IGM Protein, Gm-csf Protein, Gmcsf Protein, CSF Protein, colony stimulating factor 2 Protein, colony stimulating factor 2 (granulocyte-macrophage) Protein, CSF2 Protein, Csf2 Protein

Background: Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a cytokine which is made by activated T cells, macrophages, vascular endothelial cells and fibroblasts. It has potent stimulatory effects on the growth and differentiation of bone marrow progenitor cells that generate granulocytes monocytes/macrophages, and megakaryocytes. In peripheral tissues, GM-CSF can act on mature leukocytes to promote inflammatory responses.

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin