p300 Protein (DYKDDDDK Tag)
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- Target See all p300 (EP300) Proteins
- p300 (EP300) (E1A Binding Protein P300 (EP300))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This p300 protein is labelled with DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human EP300 / P300 (C-term DDK tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product EP300 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 20 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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An Ultraconserved Brain-Specific Enhancer Within ADGRL3 (LPHN3) Underpins Attention-Deficit/Hyperactivity Disorder Susceptibility." in: Biological psychiatry, Vol. 80, Issue 12, pp. 943-954, (2016) (PubMed).
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An Ultraconserved Brain-Specific Enhancer Within ADGRL3 (LPHN3) Underpins Attention-Deficit/Hyperactivity Disorder Susceptibility." in: Biological psychiatry, Vol. 80, Issue 12, pp. 943-954, (2016) (PubMed).
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- Target
- p300 (EP300) (E1A Binding Protein P300 (EP300))
- Alternative Name
- Ep300,p300 (EP300 Products)
- Synonyms
- KAT3B Protein, RSTS2 Protein, p300 Protein, A430090G16 Protein, A730011L11 Protein, p300 HAT Protein, E1A binding protein p300 Protein, histone acetyltransferase p300 Protein, EP300 Protein, LOC446056 Protein, ep300 Protein, Ep300 Protein
- Background
- This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.
- Molecular Weight
- 75 kDa
- NCBI Accession
- NP_001420
- Pathways
- p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
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