AKR1C4 Protein (AA 1-323) (His tag)
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- Target See all AKR1C4 Proteins
- AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-323
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This AKR1C4 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), ELISA, Western Blotting (WB), Mass Spectrometry (MS)
- Purpose
- Recombinant Human AKR1C4 Protein is produced by our E. coli expression system and the target gene encoding Met1-Tyr323 is expressed with a 6His tag at the N-terminus.
- Sequence
- MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNY RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product AKR1C4 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))
- Alternative Name
- AKR1C4 (AKR1C4 Products)
- Synonyms
- 3-alpha-HSD Protein, C11 Protein, CDR Protein, CHDR Protein, DD-4 Protein, DD4 Protein, HAKRA Protein, aldo-keto reductase family 1 member C4 Protein, AKR1C4 Protein
- Gene ID
- 1109
- UniProt
- P17516
- Pathways
- Steroid Hormone Biosynthesis
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