BTLA Protein (AA 31-134) (Fc Tag,AVI tag,Biotin)
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- Target See all BTLA Proteins
- BTLA (B and T Lymphocyte Associated (BTLA))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 31-134
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BTLA protein is labelled with Fc Tag,AVI tag,Biotin.
- Application
- Functional Studies (Func)
- Brand
- MABSol®,PrecisionAvi
- Sequence
- AA 31-134
- Specificity
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Characteristics
- This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product BTLA Protein
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- Comment
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Tris with Glycine, Arginine and NaCl, pH 7.5
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
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- Target
- BTLA (B and T Lymphocyte Associated (BTLA))
- Alternative Name
- BTLA (BTLA Products)
- Synonyms
- BTLA Protein, BTLA1 Protein, CD272 Protein, A630002H24 Protein, B and T lymphocyte associated Protein, BTLA Protein, Btla Protein
- Background
- B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
- Molecular Weight
- 40.5 kDa
- UniProt
- Q7Z6A9
- Pathways
- Cancer Immune Checkpoints
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