BTLA Protein (AA 31-150) (Fc Tag)
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- Target See all BTLA Proteins
- BTLA (B and T Lymphocyte Associated (BTLA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-150
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BTLA protein is labelled with Fc Tag.
- Purpose
- Human BTLA Protein
- Sequence
- Lys31-Ser150
- Characteristics
- Recombinant Human BTLA Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys31-Ser150.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 19.9ng/ml determined by ELISA. See testing image for detail.
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B and T Lymphocyte Associated (BTLA) (AA 31-150) protein (His-Avi Tag)
BTLA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
B and T Lymphocyte Associated (BTLA) protein (His-Avi Tag,Biotin)BTLA Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
B and T Lymphocyte Associated (BTLA) protein (mFc-His Tag)BTLA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
B and T Lymphocyte Associated (BTLA) (AA 30-176) protein (His tag)BTLA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
B and T Lymphocyte Associated (BTLA) protein (mFc Tag)BTLA Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
B and T Lymphocyte Associated (BTLA) (AA 190-289) protein (GST tag)BTLA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
B and T Lymphocyte Associated (BTLA) (AA 25-289) protein (His tag)BTLA Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func
B and T Lymphocyte Associated (BTLA) (Active) protein (Fc Tag,ECD)BTLA Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
B and T Lymphocyte Associated (BTLA) (Active) protein (Fc Tag)BTLA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
B and T Lymphocyte Associated (BTLA) (AA 179-289) protein (His tag)Crystallography grade BTLA Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- BTLA (B and T Lymphocyte Associated (BTLA))
- Alternative Name
- BTLA (BTLA Products)
- Synonyms
- BTLA Protein, BTLA1 Protein, CD272 Protein, A630002H24 Protein, B and T lymphocyte associated Protein, BTLA Protein, Btla Protein
- Background
- B- and T-lymphocyte attenuator (BTLA, CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
- Molecular Weight
- 40.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- Pathways
- Cancer Immune Checkpoints
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