BTLA Protein (His-Avi Tag,Biotin)
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- Target See all BTLA Proteins
- BTLA (B and T Lymphocyte Associated (BTLA))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BTLA protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Lys31-Ser150
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-BTLA Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product BTLA Protein
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B and T Lymphocyte Associated (BTLA) (AA 31-150) protein (Fc Tag)
BTLA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
B and T Lymphocyte Associated (BTLA) (AA 31-150) protein (His-Avi Tag)BTLA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
B and T Lymphocyte Associated (BTLA) protein (mFc-His Tag)BTLA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
B and T Lymphocyte Associated (BTLA) (AA 30-176) protein (His tag)BTLA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
B and T Lymphocyte Associated (BTLA) protein (mFc Tag)BTLA Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
B and T Lymphocyte Associated (BTLA) (AA 190-289) protein (GST tag)BTLA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
B and T Lymphocyte Associated (BTLA) (AA 25-289) protein (His tag)BTLA Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func
B and T Lymphocyte Associated (BTLA) (Active) protein (Fc Tag,ECD)BTLA Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
B and T Lymphocyte Associated (BTLA) (Active) protein (Fc Tag)BTLA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
B and T Lymphocyte Associated (BTLA) (AA 179-289) protein (His tag)Crystallography grade BTLA Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- BTLA (B and T Lymphocyte Associated (BTLA))
- Alternative Name
- BTLA (BTLA Products)
- Synonyms
- BTLA Protein, BTLA1 Protein, CD272 Protein, A630002H24 Protein, B and T lymphocyte associated Protein, BTLA Protein, Btla Protein
- Background
- CD272, BTLA, BTLA1, FLJ16065, MGC129743,B- and T-lymphocyte attenuator (BTLA, CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
- Molecular Weight
- 16.7 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.
- Pathways
- Cancer Immune Checkpoints
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