CD47 Protein (CD47) (partial) (AVI tag,His tag)

Catalog No. ABIN5608235

Product Details

Target: CD47

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: partial

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD47 protein is labelled with AVI tag,His tag.

Application: Western Blotting (WB), ELISA

Sequence: Gln 19 - Pro 139

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Endotoxin level is less than 1.0 EU per ug by the LAL method.

Biological Activity Comment:

Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha, Fc Tag at 2 μg/mL (100 uL/well) can bind Unconjugated Human CD47, His Tag, Avi Tag with a linear range of 2-12.8 ng/mL (QC tested).

Application Details

Application Notes: This recombinant protein can be used for E, WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 10 mM Bicine, pH 8.3

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.

Target Details

Target: CD47

Alternative Name: CD47 (CD47 Products)

Synonyms: IAP Protein, MER6 Protein, OA3 Protein, 9130415E20Rik Protein, AA407862 Protein, AI848868 Protein, AW108519 Protein, B430305P08Rik Protein, Itgp Protein, CD47/IAP Protein, alkaline phosphatase, intestinal Protein, CD47 molecule Protein, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) Protein, Cd47 molecule Protein, ALPI Protein, CD47 Protein, Cd47 Protein

Background: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

Molecular Weight: 16.9 kDa

Gene ID: 961

NCBI Accession: NP_942088

UniProt: Q08722