Leptin Protein (LEP)
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- Target See all Leptin (LEP) Proteins
- Leptin (LEP)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Characteristics
- Leptin Human Recombinant, expressed in E.coli
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product LEP Protein
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Leptin (LEP) (AA 22-167) protein (Fc Tag)
LEP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leptin (LEP) (Active) proteinLEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Leptin (LEP) proteinLEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) protein (His tag)LEP Origin: Goldfish Host: Yeast Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 428-635) protein (His tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leptin (LEP) (AA 22-167) protein (GST tag)LEP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Leptin (LEP) (AA 2-147) proteinLEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS
Leptin (LEP) (AA 29-164), (partial) protein (GST tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Form : Liquid. In Phosphate buffered saline, pH 7.4.
- Storage
- 4 °C
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- Target
- Leptin (LEP)
- Alternative Name
- Leptin (LEP Products)
- Synonyms
- ob Protein, obese Protein, LEPD Protein, OB Protein, OBS Protein, leptin Protein, Lep Protein, LEP Protein, lep Protein
- Background
- Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation. Recombinant human leptin was overexpressed as insoluble protein aggregate in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Synonyms: LEP, OB, OBS, Leptin, Obesity factor, Obese protein, Leptin, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Obesity, Obesity homolog mouse, Obesity Murine Homolog Leptin,. NCBI no.: NP_000221
- Molecular Weight
- 16 kDa (147 aa), confirmed by MALDI-TOF.
- Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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