GCK Protein (His tag)
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- Target See all GCK Proteins
- GCK (Glucokinase (Hexokinase 4) (GCK))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GCK protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- Hexokinase 4 Human, His-tagged, Recombinant, E.coli
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product GCK Protein
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Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (GST tag)
GCK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glucokinase (Hexokinase 4) (GCK) (AA 356-465) protein (GST tag)GCK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)GCK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glucokinase (Hexokinase 4) (GCK) proteinGCK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (Strep Tag)Crystallography grade GCK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (Strep Tag)Crystallography grade GCK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl pH8.0, 10% glycerol.
- Storage
- 4 °C
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- Target
- GCK (Glucokinase (Hexokinase 4) (GCK))
- Alternative Name
- Hexokinase 4 (GCK Products)
- Synonyms
- FGQTL3 Protein, GK Protein, GLK Protein, HHF3 Protein, HK4 Protein, HKIV Protein, HXKP Protein, LGLK Protein, MODY2 Protein, Gk Protein, Gls006 Protein, GCK Protein, BA4487 Protein, glk Protein, hk4 Protein, hhf3 Protein, hkiv Protein, hxkp Protein, gck-A Protein, mody2 Protein, glucokinase Protein, GLUKA Protein, RNGK2 Protein, glucokinase Protein, glucokinase (hexokinase 4) L homeolog Protein, glucokinase (hexokinase 4) Protein, GCK Protein, Gck Protein, glcK Protein, Bphyt_1025 Protein, Halhy_6548 Protein, gk Protein, gck.L Protein, gck Protein
- Background
- Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase4 is found in the liver and pancreatic beta-cells, where it is controlled by insulin (activation) and glucagon (inhibition). In pancreatic beta-cells, type IV enzyme acts as a glucose sensor to modify insulin secretion. Hexokinase4 is commonly called ‘glucokinase’. Recombinant human Hexokinase4, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: GCK, Hexokinase 4, EC 2.7.1.2, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, Glucokinase, ATP D hexose 6 phosphotransferase, GK, GLK, Glucokinase, Glucokinase isoform 1, Hexokinase 4, Hexokinase D, Hexokinase D pancreatic isozyme, Hexokinase4, HexokinaseD, HHF 3, HHF3, HK 4, HXKP, Maturity onset diabetes of the young 2, MODY 2, MODY2. NCBI no.: NP_000153
- Molecular Weight
- 54.3kDa (485aa)
- Pathways
- MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
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