×
For best experience we recommend to activate Javascript in your browser.
NLRP3 antibody (Pyrin Domain)
NLRP3
Reactivity: Human, Mouse
WB, IHC, IF, IP
Host: Mouse
Monoclonal
Cryo-2
unconjugated
Product Details anti-NLRP3 Antibody
(hide)
Key Features
NLRP3 antibody for reliable recognition of mouse and human NLRP3/NALP3
This un-conjugated monoclonal antibody has 60 PubMed citations
Target
See all NLRP3 Antibodies
NLRP3
(NLR Family, Pyrin Domain Containing 3 (NLRP3))
Binding Specificity
All epitopes for NLRP3 antibodies
AA 1-93, Pyrin Domain
Reactivity
All reactivities for NLRP3 antibodies
Human, Mouse
Host
All hosts for NLRP3 antibodies
Mouse
Clonality
All clonalities for NLRP3 antibodies
Monoclonal
Conjugate
All conjugates for NLRP3 antibodies
This NLRP3 antibody is un-conjugated
Application
All applications for NLRP3 antibodies
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Specificity
Recognizes mouse and human NLRP3/NALP3.
Cross-Reactivity
Human, Mouse (Murine)
Purification
Purified from concentrated hybridoma tissue culture supernatant.
Purity
>95 % (SDS-PAGE)
Immunogen
Recombinant mouse NLRP3/NALP3 (pyrin domain) (aa 1-93).
Clone
Cryo-2
Isotype
IgG2b
Alternatives
(show)
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 15-120) antibody
NLRP3
Reactivity: Human, Mouse, Rat, Dog
WB, ELISA, FACS, IHC (p), IF (p), ICC, IF (cc), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Details
(hide)
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Handling
(hide)
Format
Liquid
Concentration
Lot specific
Buffer
In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Short Term Storage: +4°C Long Term Storage: -20°C Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date
12 months
References for anti-NLRP3 antibody (ABIN1169100)
(hide)
Zhu, Tang : "LncRNA Gm14205 induces astrocytic NLRP3 inflammasome activation via inhibiting oxytocin receptor in postpartum depression. " in: Bioscience reports , Vol. 40 , Issue 8 , (2020 ) (PubMed ).
Wong, Lewis, Doherty, Shi, Cashikar, Amelianchik, Tymchuk, Sullivan, Qian, Covey, Petsko, Holtzman, Paul, Luo : "25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner. " in: Journal of neuroinflammation , Vol. 17 , Issue 1 , pp. 192 , (2020 ) (PubMed ).
Hoyle, Redondo-Castro, Cook, Tzeng, Allan, Brough, Lemarchand : "Hallmarks of NLRP3 inflammasome activation are observed in organotypic hippocampal slice culture. " in: Immunology , Vol. 161 , Issue 1 , pp. 39-52 , (2020 ) (PubMed ).
Shrivastava, Visoso-Carvajal, Garcia-Cordero, Leon-Juarez, Chavez-Munguia, Lopez, Nava, Villegas-Sepulveda, Cedillo-Barron : "Dengue Virus Serotype 2 and Its Non-Structural Proteins 2A and 2B Activate NLRP3 Inflammasome. " in: Frontiers in immunology , Vol. 11 , pp. 352 , (2020 ) (PubMed ).
Wang, Hu, Wu, Feng, Li, Liu, Wang, Chen, Tian, Xiao, Zhang, Shereen, Chen, Pan, Wan, Wu, Wu : "STING promotes NLRP3 localization in ER and facilitates NLRP3 deubiquitination to activate the inflammasome upon HSV-1 infection. " in: PLoS pathogens , Vol. 16 , Issue 3 , pp. e1008335 , (2020 ) (PubMed ).
Lai, Hsu, Lee, Chiang : "Microtubule-Mediated NLRP3 Inflammasome Activation Is Independent of Microtubule-Associated Innate Immune Factor GEF-H1 in Murine Macrophages. " in: International journal of molecular sciences , Vol. 21 , Issue 4 , (2020 ) (PubMed ).
Wooff, Fernando, Wong, Dietrich, Aggio-Bruce, Chu-Tan, Robertson, Doyle, Man, Natoli : "Caspase-1-dependent inflammasomes mediate photoreceptor cell death in photo-oxidative damage-induced retinal degeneration. " in: Scientific reports , Vol. 10 , Issue 1 , pp. 2263 , (2020 ) (PubMed ).
Svensson, Andersson, Manouchehrian, Yang, Deierborg : "Voluntary running does not reduce neuroinflammation or improve non-cognitive behavior in the 5xFAD mouse model of Alzheimer's disease. " in: Scientific reports , Vol. 10 , Issue 1 , pp. 1346 , (2020 ) (PubMed ).
Cai, Ge, Mennone, Hoque, Ouyang, Boyer : "Inflammasome Is Activated in the Liver of Cholestatic Patients and Aggravates Hepatic Injury in Bile Duct-Ligated Mouse. " in: Cellular and molecular gastroenterology and hepatology , Vol. 9 , Issue 4 , pp. 679-688 , (2020 ) (PubMed ).
Dionísio, Oliveira, Gaspar, Gama, Castro-Caldas, Amaral, Rodrigues : "Ablation of RIP3 protects from dopaminergic neurodegeneration in experimental Parkinson's disease. " in: Cell death & disease , Vol. 10 , Issue 11 , pp. 840 , (2020 ) (PubMed ).
Du, Lu, Wang, Ding, Wu, Hu : "The pore-forming subunit Kir6.1 of the K-ATP channel negatively regulates the NLRP3 inflammasome to control insulin resistance by interacting with NLRP3. " in: Experimental & molecular medicine , Vol. 51 , Issue 8 , pp. 1-13 , (2020 ) (PubMed ).
Hoss, Mueller, Rojas Ringeling, Rodriguez-Alcazar, Brinkschulte, Seifert, Stahl, Broderick, Putnam, Kolodner, Canzar, Geyer, Hoffman, Latz : "Alternative splicing regulates stochastic NLRP3 activity. " in: Nature communications , Vol. 10 , Issue 1 , pp. 3238 , (2020 ) (PubMed ).
Sharif, Wang, Wang, Magupalli, Andreeva, Qiao, Hauenstein, Wu, Núñez, Mao, Wu : "Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome. " in: Nature , Vol. 570 , Issue 7761 , pp. 338-343 , (2020 ) (PubMed ).
Xue, Xi, Liu, Guo, Zhang, Zhang, Li, Yang, Zhou, Yang, Zhang, Zhang, Gu, Yang, Da, Yao, Duo, Zhang : "miR-21 promotes NLRP3 inflammasome activation to mediate pyroptosis and endotoxic shock. " in: Cell death & disease , Vol. 10 , Issue 6 , pp. 461 , (2020 ) (PubMed ).
Huang, Feng, Jiang, Li, Xiang, Guo, Yang, Fei, Guo, Zheng, Wu, Chen : "VSIG4 mediates transcriptional inhibition of Nlrp3 and Il-1β in macrophages. " in: Science advances , Vol. 5 , Issue 1 , pp. eaau7426 , (2020 ) (PubMed ).
Liu, Lu, Chen, Muthuraj, Li, Pattabiraman, Zempleni, Kachman, Natarajan, Yu : "Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice. " in: Nutrients , Vol. 12 , Issue 2 , (2020 ) (PubMed ).
Deng, Guo, Tam, Johnson, Brickey, New, Lenox, Shi, Golenbock, Koller, McKinnon, Beutler, Ting : "Platelet-activating factor (PAF) mediates NLRP3-NEK7 inflammasome induction independently of PAFR. " in: The Journal of experimental medicine , Vol. 216 , Issue 12 , pp. 2838-2853 , (2020 ) (PubMed ).
Sanchez-Lopez, Zhong, Stubelius, Sweeney, Booshehri, Antonucci, Liu-Bryan, Lodi, Terkeltaub, Lacal, Murphy, Hoffman, Tiziani, Guma, Karin : "Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production. " in: Cell metabolism , Vol. 29 , Issue 6 , pp. 1350-1362.e7 , (2020 ) (PubMed ).
Kiyohara, Sujino, Teratani, Miyamoto, Arai, Nomura, Harada, Aoki, Koda, Mikami, Mizuno, Naganuma, Hisamatsu, Kanai : "Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells. " in: Cellular and molecular gastroenterology and hepatology , Vol. 7 , Issue 1 , pp. 135-156 , (2019 ) (PubMed ).
Hafner-Bratkovič, Sušjan, Lainšček, Tapia-Abellán, Cerović, Kadunc, Angosto-Bazarra, Pelegrin, Jerala : "NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway. " in: Nature communications , Vol. 9 , Issue 1 , pp. 5182 , (2019 ) (PubMed ).
Target Details for NLRP3
(hide)
Target
NLRP3
(NLR Family, Pyrin Domain Containing 3 (NLRP3))
Alternative Name
NLRP3/NALP3 (NLRP3 Products )
Synonyms
CIAS1 antibody, Nalp3 antibody, avp antibody, cias1 antibody, nalp3 antibody, pypaf1 antibody, agtavprl antibody, AGTAVPRL antibody, AII antibody, AVP antibody, C1orf7 antibody, CLR1.1 antibody, FCAS antibody, FCU antibody, MWS antibody, NALP3 antibody, PYPAF1 antibody, AII/AVP antibody, Cias1 antibody, Mmig1 antibody, Pypaf1 antibody, NLR family pyrin domain containing-like antibody, NLR family pyrin domain containing 3 antibody, NLR family, pyrin domain containing 3 L homeolog antibody, NLR family, pyrin domain containing 3 antibody, NLRPL antibody, NLRP3 antibody, nlrp3.L antibody, Nlrp3 antibody
Background
The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).
UniProt
Q8R4B8
Pathways
Cellular Response to Molecule of Bacterial Origin , Positive Regulation of Endopeptidase Activity , Inflammasome
Recently viewed
(hide)