Histone 3 antibody
(2 references)-
- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Reactivity
- Tomato, Arabidopsis thaliana, Zea mays, Moss, Chlamydomonas reinhardtii
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunocytochemistry (ICC)
- Predicted Reactivity
- dicots including: Vitis vinifera, monocots: Hordeum vulgare, Oryza sativa, Zea mays, trees: Pinus pinaster, algae: Volvox sp.
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 15 / 17 kDa
- Purification
- serum
- Immunogen
- KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At4g10980), H3.2 P59226(At4g09200,At4g27360,At4g10390,At4g10400,At4g65360), H3-like 2 Q9FXI7 (At4g19890)
- Top Product
- Discover our top product H3 Primary Antibody
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anti-Histone 3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27me) antibodyH3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (C-Term) antibodyH3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Recommended Dilution: 1 : 5000 with standard ECL (WB), 1 : 100 - 1 : 500 (ICC)
- Comment
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Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
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: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).
: "A maize cystatin suppresses host immunity by inhibiting apoplastic cysteine proteases." in: The Plant cell, Vol. 24, Issue 3, pp. 1285-300, (2012) (PubMed).
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: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).
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- Target
- Histone 3 (H3)
- Alternative Name
- H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- Background
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AGI Code: At4g40030
Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4. - Molecular Weight
- expected: 15 kDa, apparent: 17 kDa
- UniProt
- P59169, P59226, Q9FXI7
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