XRCC6 antibody (AA 506-541)
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- Target See all XRCC6 Antibodies
- XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))
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Binding Specificity
- AA 506-541
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Reactivity
- Human, Mouse, Monkey, Xenopus laevis, Hamster
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This XRCC6 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
- Purification
- Concentrated. Purified from the ascites fluid by Protein A chromatography
- Immunogen
- Human placental extract designated as PSE1-PL
- Clone
- N3H10
- Isotype
- IgG2b
- Top Product
- Discover our top product XRCC6 Primary Antibody
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- Application Notes
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Immunofluorescence
Immunoprecipitation (Native and denatured)(Use Protein A) (Ab 2µg/mg protein lysate)
Western blotting: (0.25-0.5 µg/ml for 2hrs at RT)
Immunohistology (Formalinparaffin)(Ab 1-2g/ml for 30 minute at RT)
Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH 6.0. - Comment
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Cellular Localization: Nuclear.
Recommended Positive Control: BT474, HEP-G-2 or HeLa Cells, Tonsil - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL.
- Buffer
- 10mM PBS, pH 7.4, with 0.2% BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens." in: Molecular biology reports, Vol. 18, Issue 1, pp. 15-28, (1993) (PubMed).
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Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens." in: Molecular biology reports, Vol. 18, Issue 1, pp. 15-28, (1993) (PubMed).
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- Target
- XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))
- Alternative Name
- Ku (p70) (XRCC6 Products)
- Synonyms
- CTC75 antibody, CTCBF antibody, G22P1 antibody, KU70 antibody, ML8 antibody, TLAA antibody, 70kDa antibody, G22p1 antibody, Ku70 antibody, Kup70 antibody, X-ray repair cross complementing 6 antibody, ATP-dependent DNA helicase II, 70 kDa subunit antibody, X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog antibody, X-ray repair complementing defective repair in Chinese hamster cells 6 antibody, XRCC6 antibody, Bm1_41430 antibody, xrcc6.L antibody, Xrcc6 antibody
- Background
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The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Also available without BSA and azide at 1mg/ml. - Molecular Weight
- 70kDa
- Gene ID
- 2547
- Pathways
- DNA Damage Repair
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