Retinoblastoma 1 antibody (pSer795)

Catalog No. ABIN3201012

Product Details anti-Retinoblastoma 1 Antibody

Target: Retinoblastoma 1 (RB1)

Binding Specificity: pSer795

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Specific to human Rb phosphorylated at Ser795.

Cross-Reactivity (Details): Not tested for other species. Can detect endogenous levels of Rb phosphorylated at Ser795 in most cell lines.

Purification: Purified

Sterility: Sterile filtered

Immunogen: A synthetic peptide containing pSer795 of human Rb

Clone: 28B5

Isotype: IgG2a kappa

Application Details

Application Notes: 1. Western blotting: ~1 g/mL
2. ELISA
Other applications were not tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C

Storage Comment: Store at -20 C (For long term storage: -70 C).

References for anti-Retinoblastoma 1 antibody (ABIN3201012)

Sherr: "Cancer cell cycles." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1672-7, (1997) (PubMed).

Welch, Wang: "A C-terminal protein-binding domain in the retinoblastoma protein regulates nuclear c-Abl tyrosine kinase in the cell cycle." in: Cell, Vol. 75, Issue 4, pp. 779-90, (1993) (PubMed).

Nevins: "E2F: a link between the Rb tumor suppressor protein and viral oncoproteins." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 424-9, (1992) (PubMed).

Hu, Dyson, Harlow: "The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations." in: The EMBO journal, Vol. 9, Issue 4, pp. 1147-55, (1990) (PubMed).

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: RB (RB1 Products)

Synonyms: OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody

Background: Retinoblastoma protein (Rb), the tumor suppressor product of the retinoblastoma susceptibility gene, is a 110 kDa protein that functions as a negative regulator of the cell cycle by arresting cells in the G1 phase and halting inappropriate cell proliferation. At the transcriptional level, Rb protein exerts its growth suppressive function by binding to transcription factors including E2F1, PU1, ATF2, UBF, Elf-1 and c-Abl. Loss of Rb function leads to uncontrolled cell growth and tumor development and is found in all retinoblastomas and in a variety of other human malignancies. The ability of Rb protein to inhinbit transcription and cell cycle progression is inactivated by hosphorylation, which is catalyzed by the cyclin-dependent protein kinases.

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy