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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||13 Antibodies||57 Antibodies|
|Immunochromatography (IC)||1 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||3 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||28 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Antigen||Angiopoietin 1 (ANGPT1) Antibodies|
|Epitope||AA 225-239, Middle Region Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This Angiopoietin 1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Product Details anti-Angiopoietin 1 AntibodyTarget Details Angiopoietin 1 Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Angiopoietin-1(ANGPT1) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Gene Name: angiopoietin 1
Protein Name: Angiopoietin-1
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Angiopoietin 1(225-239aa RQTYIIQELEKQLNR), different from the related mouse and rat sequences by two amino acids.|
Target Details Angiopoietin 1Product Details anti-Angiopoietin 1 Antibody Application Details Handling Images back to top
|Alternative Name||ANGPT1 (ANGPT1 Antibody Abstract)|
|Background||Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Endothelial Tie2/Tek ligands angiopoietin-1(ANGPT1) and angiopoietin-2(ANGPT2): regional localization of the human genes to 8q22.3-q23 and 8p23.|
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process|
Application DetailsProduct Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details Handling back to top