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anti-Human Angiopoietin 1 antibody for ELISA

Recommended Angiopoietin 1 Antibody (supplied by: Log in to see )

Antigen
Angiopoietin 1 (ANGPT1) Antibodies
  • 1110046O21Rik
  • AGP1
  • AGPT
  • Agpt
  • Agpt1
  • Ang-1
  • ANG-1
  • ANG1
  • Ang1
  • ang1
  • angpt1
  • DHCR14A
  • MGC80788
  • NET47
  • wu:fc76g06
Epitope
N-Term, AA 21-40
26
17
13
10
9
8
7
6
5
5
5
5
5
4
4
3
3
3
3
2
2
1
1
Reactivity
Human, Mouse (Murine)
125
61
36
18
16
5
2
1
Host
Rabbit
144
15
8
1
Clonality
Polyclonal
Conjugate
This Angiopoietin 1 antibody is un-conjugated
17
14
7
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
125
81
27
25
11
11
8
5
3
2
2
Supplier
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Catalog No. ABIN269766
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012704609 ABIN2464478 ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012704609 ABIN3044373 IHC (p) ELISA WB Rabbit IgG AA 225-239, Middle Region Log in to see Polyclonal
0.0012704609 ABIN962636 IHC (p) ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012704609 ABIN790997 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.0012704609 ABIN2470306 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012704609 ABIN1171921 IHC ELISA WB Biotin Rabbit IgG AA 284-452 Log in to see Polyclonal
0.0012704609 ABIN1857989 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 284-452 Log in to see
0.0012704609 ABIN2616204 IHC (p) ELISA WB Rabbit IgG AA 225-239 Log in to see Polyclonal
0.0012704609 ABIN768552 ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012704609 ABIN1892881 ELISA WB APC Rabbit IgG C-Term, AA 378-410 Log in to see Polyclonal
0.0012704609 ABIN1892883 ELISA WB Biotin Rabbit IgG C-Term, AA 378-410 Log in to see Polyclonal
0.0012704609 ABIN1892882 ELISA WB FITC Rabbit IgG C-Term, AA 378-410 Log in to see Polyclonal
0.0012704609 ABIN1892880 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 378-410 Log in to see Polyclonal
0.0012704609 ABIN1892884 ELISA WB PE Rabbit IgG C-Term, AA 378-410 Log in to see Polyclonal
0.0012704609 ABIN1857991 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 21-228 Log in to see Polyclonal
0.0012704609 ABIN1452921 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012704609 ABIN1892879 ELISA WB HRP Rabbit IgG C-Term, AA 378-410 Log in to see Polyclonal
0.0012704609 ABIN3181025 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.0012704609 ABIN3183282 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012704609 ABIN1171919 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 284-452 Log in to see Polyclonal

Top referenced anti-Angiopoietin 1 antibody for ELISA

Similar anti-Angiopoietin 1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 26 Antibodies 56 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Immunochromatography (IC) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 6 Antibodies
Immunofluorescence (IF) 3 Antibodies 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 13 Antibodies 22 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Western Blotting (WB) 43 Antibodies

General

Antigen Angiopoietin 1 (ANGPT1) Antibodies
Epitope N-Term, AA 21-40
(26), (17), (13), (10), (9), (8), (7), (6), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine)
(125), (61), (36), (18), (16), (5), (2), (1)
Host Rabbit
(144), (15), (8), (1)
Clonality
Polyclonal
Conjugate This Angiopoietin 1 antibody is un-conjugated
(17), (14), (7), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(125), (81), (27), (25), (11), (11), (8), (5), (3), (2), (2)
Pubmed 1 reference available
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Product Details anti-Angiopoietin 1 Antibody

Target Details Angiopoietin 1 Application Details Handling References for anti-Angiopoietin 1 Antibody (ABIN269766) Images
Specificity This antiserum is directed against mouse angiopoietin 1 and shows no reactivity with mouse angiopoietin 2. A 57 kDa band is detected when assayed by immunoblot. This reagent cross-reacts with human angiopoietin 1. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.
Cross-Reactivity (Details) Partially cross reactivity is noted with human angiopoietin . Not yet tested in other species.
Purification Whole antisera
Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Isotype IgG

Target Details Angiopoietin 1

Product Details anti-Angiopoietin 1 Antibody Application Details Handling References for anti-Angiopoietin 1 Antibody (ABIN269766) Images back to top
Antigen
Alternative Name Angiopoietin-1 (ANGPT1 Antibody Abstract)
Background Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of theendothelium, by regulating tyrosine phosphorylation of the membrane receptorTie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potentialfibrinogen-like domain at the carboxy terminus and coiled-coil regions in the aminoterminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle,mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. Inthe adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is thefirst time that an Ang antibody recognises a band above 55 kD. This antibody gives aband at 75 kD which resembles the original size, because these are soluble highlyglycosylated proteins, which should run higher than the calculated molecular weight of55 kD.
Gene Symbol: ANGPT1
Gene ID 284
Research Area Growth Factors
Pathways RTK Signaling

Application Details

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Handling References for anti-Angiopoietin 1 Antibody (ABIN269766) Images back to top
Application Notes Suitable for western blotting, Immunohistochemistry and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background Due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close - their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.
Recommended dilutions: ELISA 1:5000-1:25000, Immunohistochemistry 1:200-1:800, Immunohistochemistry-Paraffin 1:200-1:800, Western Blot 1:500-1:2000
Restrictions For Research Use only

Handling

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details References for anti-Angiopoietin 1 Antibody (ABIN269766) Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate with 0.15 M Sodium Chloride pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

References for anti-Angiopoietin 1 Antibody (ABIN269766)

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details Handling Images back to top
Product cited in:

Chade, Krier, Textor, Lerman, Lerman: "Endothelin-a receptor blockade improves renal microvascular architecture and function in experimental hypercholesterolemia." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 12, pp. 3394-403, 2006

Images

Product Details anti-Angiopoietin 1 Antibody Target Details Angiopoietin 1 Application Details Handling References for anti-Angiopoietin 1 Antibody (ABIN269766) back to top
Supplier Images
Western Blotting (WB) image for anti-Angiopoietin 1 antibody (ANGPT1) (N-Term) (ABIN269766) anti-Angiopoietin 1 (ANGPT1) (AA 21-40), (N-Term) antibody