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anti-Human GRB2 antibody for Immunohistochemistry

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor-bound protein 2
  • Growth factor receptor-bound protein 2
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • LOC100342500
  • grb2b
Epitope
C-Term
32
18
16
7
6
6
5
5
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
154
63
62
16
9
8
7
5
4
3
3
3
3
2
2
2
2
1
1
Host
Goat
102
28
23
2
Clonality
Polyclonal
Conjugate
This GRB2 antibody is un-conjugated
7
4
3
3
3
3
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
137
67
54
30
13
7
5
4
2
2
2
1
Options
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Catalog No. ABIN184670
$ 326.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.142917 ABIN1532105 IHC ELISA WB Rabbit IgG pSer159 Log in to see Polyclonal 2
9.142917 ABIN257708 ELISA IHC IHC (p) WB Goat C-Term, both Isoforms Log in to see Polyclonal 1
9.142917 ABIN2465225 IHC ELISA WB Goat C-Term Log in to see Polyclonal
9.142917 ABIN3031112 IHC ELISA WB Rabbit Ig Fraction AA 187-216 Log in to see Polyclonal
9.142917 ABIN3031114 IHC ELISA WB Rabbit Ig Fraction AA 89-118 Log in to see Polyclonal
1 ABIN967754 BI IP IHC WB Mouse IgG1 AA 1-217 Log in to see 81-GRB2 5
1 ABIN967755 BI IP IHC WB Mouse IgG1 AA 1-217 Log in to see 81-GRB2 5
1 ABIN1876890 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1859065 ICC IHC IP WB Rabbit IgG AA 1-217 Log in to see Polyclonal
1 ABIN1954046 IHC ELISA WB APC Rabbit IgG Tyr209 Log in to see Polyclonal
1 ABIN1955298 IHC ELISA WB Biotin Rabbit IgG Tyr209 Log in to see Polyclonal
1 ABIN1953174 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Tyr209 Log in to see Polyclonal
1 ABIN1956479 IHC ELISA WB FITC Rabbit IgG Tyr209 Log in to see Polyclonal
1 ABIN1958819 IHC ELISA WB PE Rabbit IgG Tyr209 Log in to see Polyclonal
1 ABIN2562931 IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal
1 ABIN1957674 IHC ELISA WB HRP Rabbit IgG Tyr209 Log in to see Polyclonal
1 ABIN2621498 ELISA IHC WB Rabbit IgG Tyr209 Log in to see Polyclonal
1 ABIN2406480 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2276940 IHC ELISA WB FITC Rabbit IgG Tyr209 Log in to see Polyclonal
1 ABIN2276942 IHC ELISA WB PE Rabbit IgG Tyr209 Log in to see Polyclonal

Top referenced anti-GRB2 antibody for Immunohistochemistry

Similar anti-GRB2 Antibodies

Application / Reactivity Mouse (Murine) Human
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 24 Antibodies 68 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 5 Antibodies
Immunofluorescence (IF) 9 Antibodies 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 55 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope C-Term
(32), (18), (16), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(154), (63), (62), (16), (9), (8), (7), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Host Goat
(102), (28), (23), (2)
Clonality
Polyclonal
Conjugate This GRB2 antibody is un-conjugated
(7), (4), (3), (3), (3), (3)
Method Type Peptide ELISA
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(137), (67), (54), (30), (13), (7), (5), (4), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN184670) Images
Blocking Peptide Blocking peptide for this product available: ABIN369579
Specificity This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling References for anti-GRB2 Antibody (ABIN184670) Images back to top
Antigen
Alternative Name GRB2 (GRB2 Antibody Abstract)
Background Synonyms: GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, abundant SRC homology
Gene ID 2885, 14784, 81504
NCBI Accession NP_987102, NP_002077
Research Area Cancer
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling References for anti-GRB2 Antibody (ABIN184670) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30 kDa band seen in Jurkat and NIH3T3 lysates. Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Recommended concentration: 0.1-0.3 µg/mL. Also islets of Langerhans in paraffin embedded Human Pancreas is specifically stained (please contact technical support for details).
Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details References for anti-GRB2 Antibody (ABIN184670) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-GRB2 Antibody (ABIN184670)

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling Images back to top
Background publications

Lowenstein, Daly, Batzer, Li, Margolis, Lammers, Ullrich, Skolnik, Bar-Sagi, Schlessinger: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN184670) back to top
Supplier Images
Western Blotting (WB) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN184670) ABIN184670 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per la...
Immunohistochemistry (IHC) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN184670) ABIN184670 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen ...