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anti-Pig (Porcine) GRB2 antibody for Western Blotting

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor-bound protein 2
  • Growth factor receptor-bound protein 2
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • LOC100342500
  • grb2b
Epitope
C-Term, both Isoforms
36
18
15
7
6
6
6
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
158
49
49
11
9
5
3
2
2
2
2
1
1
Host
Goat
104
30
25
2
Clonality
Polyclonal
Conjugate
This GRB2 antibody is un-conjugated
7
4
3
3
3
3
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
135
69
53
30
10
8
5
3
3
2
1
Supplier
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Available images

Catalog No. ABIN257708
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590793 ABIN2435838 IHC WB Goat Log in to see Polyclonal
1 ABIN3198099 WB Rabbit Log in to see Polyclonal
1 ABIN2972725 WB Rabbit Center Log in to see Polyclonal

Top referenced anti-GRB2 antibody for Western Blotting

Similar anti-GRB2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Pig (Porcine)
Western Blotting (WB) 133 Antibodies 45 Antibodies 45 Antibodies 4 Antibodies
Immunoprecipitation (IP) 8 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies 6 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 54 Antibodies 13 Antibodies 12 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunofluorescence (IF) 10 Antibodies

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope C-Term, both Isoforms
(36), (18), (15), (7), (6), (6), (6), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(158), (49), (49), (11), (9), (5), (3), (2), (2), (2), (2), (1), (1)
Host Goat
(104), (30), (25), (2)
Clonality
Polyclonal
Conjugate This GRB2 antibody is un-conjugated
(7), (4), (3), (3), (3), (3)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(135), (69), (53), (30), (10), (8), (5), (3), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN257708) Images
Specificity This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Cross-Reactivity (Details) Expected cross reactivity from sequence similarity: Mouse, Rat, Dog,Cow.
Purification Immunogen affinity purified
Immunogen Peptide with sequence C-PRNYVTPVNRNV corresponding to C-Terminus according to NP_987102.1.

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling References for anti-GRB2 Antibody (ABIN257708) Images back to top
Antigen
Alternative Name GRB2 (GRB2 Antibody Abstract)
Background Gene Symbol: GRB2
Gene ID 2885
Research Area Cancer
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling References for anti-GRB2 Antibody (ABIN257708) Images back to top
Application Notes Western Blot 0.05 - 0.1.5 μg/mL, Immunohistochemistry 0.1 - 0.3 μg/mL, Peptide ELISA Detection limit 1:32000WB: Approx. 26 kDa band observed in MOLT4 lysates, of human thymus and of human, mouse, rat, porcine brain and spleen lysates (calculated MW of 25.2 kDa band according to human NP_002077.1 and according to rat NP_110473.2). IHC-P: Human tonsil stains mantle zone and to a lessser extent germinal centre. Data obtained by previous batch (different goat). Islets of Langerhans of human pancreas is specifically stained.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details References for anti-GRB2 Antibody (ABIN257708) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-GRB2 Antibody (ABIN257708)

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling Images back to top
Product cited in:

Lowenstein, Daly, Batzer, Li, Margolis, Lammers, Ullrich, Skolnik, Bar-Sagi, Schlessinger: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN257708) back to top
Supplier Images
Western Blotting (WB) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN257708) ABIN257708 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per la...
Immunohistochemistry (IHC) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN257708) ABIN257708 (0.3?g/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN257708) Immunohistochemistry-Paraffin: GRB2 Antibody [ABIN257708] - Analysis of human tonsil....
Western Blotting (WB) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN257708) Western Blot: GRB2 Antibody [ABIN257708] - Analysis of Human (A) and Pig (B) Spleen l...
Western Blotting (WB) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN257708) Western Blot: GRB2 Antibody [ABIN257708] - Analysis of Human Thymus (A) and Molt4 (B)...
Western Blotting (WB) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN257708) Western Blot: GRB2 Antibody [ABIN257708] - Analysis of Mouse (A+C) and Rat (B+D) Brai...