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anti-Human PDGFA antibody for Enzyme Immunoassay

Recommended PDGFA Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
  • pdgf
  • PDGF-A
  • pdgf-a
  • PDGF1
  • pdgf1
  • pdgfa
  • pdgfa-2
  • PDGFACP
  • zgc:100777
Reactivity
Human
88
38
37
2
1
1
1
Host
Rabbit
78
6
5
3
Clonality
Polyclonal
Conjugate
This PDGFA antibody is conjugated to Biotin
6
5
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
65
35
31
22
17
6
4
3
3
2
2
2
2
1
1
Supplier
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Catalog No. ABIN116206
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008854307 ABIN116205 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0008854307 ABIN116207 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
0.0008854307 ABIN116208 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal

Similar anti-PDGFA Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 33 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 65 Antibodies

General

Antigen Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
Reactivity Human
(88), (38), (37), (2), (1), (1), (1)
Host Rabbit
(78), (6), (5), (3)
Clonality
Polyclonal
Conjugate This PDGFA antibody is conjugated to Biotin
(6), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(65), (35), (31), (22), (17), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1)
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Product Details anti-PDGFA Antibody

Target Details PDGFA Application Details Handling Images
Specificity Human Platelet Derived Growth Factor AA (hPDGF-AA).
Purification Affinity Chromatography
Immunogen Highly pure (>98%) E.coli derived recombinant Human PDGF-AA (Cat.-No PA118)

Target Details PDGFA

Product Details anti-PDGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDGFA (PDGFA Antibody Abstract)
Background PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFRalpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A
Gene ID 5154
NCBI Accession NP_002598
UniProt P04085
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDGFA Antibody Target Details PDGFA Handling Images back to top
Application Notes ELISA: To detect hPDGF-AA by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-AA is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDGFA Antibody Target Details PDGFA Application Details Images back to top
Reconstitution Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.