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|Antigen||Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies|
|Conjugate||This PDGFA antibody is conjugated to Biotin Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-PDGFA AntibodyTarget Details PDGFA Application Details Handling Images
|Specificity||Human Platelet Derived Growth Factor AA (hPDGF-AA).|
|Immunogen||Highly pure (>98%) E.coli derived recombinant Human PDGF-AA (Cat.-No PA118)|
Target Details PDGFAProduct Details anti-PDGFA Antibody Application Details Handling Images back to top
|Alternative Name||PDGFA (PDGFA Antibody Abstract)|
|Background||PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFRalpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling|
Application DetailsProduct Details anti-PDGFA Antibody Target Details PDGFA Handling Images back to top
ELISA: To detect hPDGF-AA by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-AA is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDGFA Antibody Target Details PDGFA Application Details Images back to top
|Reconstitution||Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives|
|Handling Advice||Avoid repeated freezing and thawing. Centrifuge vial prior to opening!|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.|