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anti-Parapoxvirus PDGFC antibody for Western Blotting

Recommended PDGFC Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor C (PDGFC) Antibodies
  • 1110064L01Rik
  • AI647969
  • FALLOTEIN
  • PDGF-C
  • SCDGF
Reactivity
Parapoxvirus
60
36
19
Host
Rabbit
61
4
3
2
Clonality
Polyclonal
Conjugate
This PDGFC antibody is un-conjugated
5
5
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
47
40
26
11
10
4
3
3
1
1
Supplier
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Catalog No. ABIN1589843
$ 242.55
Plus shipping costs $45.00

Similar anti-PDGFC Antibodies

Application / Reactivity Parapoxvirus
Western Blotting (WB) 1 Antibodies

General

Antigen Platelet-Derived Growth Factor C (PDGFC) Antibodies
Reactivity Parapoxvirus
(60), (36), (19)
Host Rabbit
(61), (4), (3), (2)
Clonality
Polyclonal
Conjugate This PDGFC antibody is un-conjugated
(5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(47), (40), (26), (11), (10), (4), (3), (3), (1), (1)
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Product Details anti-PDGFC Antibody

Target Details PDGFC Application Details Handling Images
Specificity Recombinant ov-VEGF-E
Characteristics Produced from sera of rabbits immunized with highly pure recombinant ov-VEGF-E produced in E. coli. A DNA sequence encoding the mature variant of ov-VEGF-E isolate D1701 (Dehio et al., 1999, GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight Cysteine residues of the central Cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999, Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E cannot bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor -2/KDR.
Isotype IgG

Target Details PDGFC

Product Details anti-PDGFC Antibody Application Details Handling Images back to top
Antigen
Alternative Name VEGF-E (PDGFC Antibody Abstract)
Background A DNA sequence encoding the mature variant of ov-VEGF-E isolate D1701 was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight Cysteine residues of the central Cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins. Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E cannot bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.
Synonyms: VEGF-E
UniProt Q9YMF3
Pathways RTK Signaling

Application Details

Product Details anti-PDGFC Antibody Target Details PDGFC Handling Images back to top
Application Notes Western Blot: use 1-5 µg/mL
Comment

Rabbit IG Polyclonal Antibody

Restrictions For Research Use only

Handling

Product Details anti-PDGFC Antibody Target Details PDGFC Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS
Handling Advice Centrifuge vial prior to opening.
Storage -20 °C
Storage Comment The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.
Expiry Date 6 months