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|Antigen||Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This PDGFRA antibody is un-conjugated Alternatives|
Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
|3 references available|
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Product Details anti-PDGFRA AntibodyTarget Details PDGFRA Application Details Handling References for anti-PDGFRA antibody (ABIN967626) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Mouse PDGF Receptor alpha chain|
Target Details PDGFRAProduct Details anti-PDGFRA Antibody Application Details Handling References for anti-PDGFRA antibody (ABIN967626) Images back to top
|Alternative Name||CD140a (PDGFRA Antibody Abstract)|
The APA5 antibody reacts with Platelet-Derived Growth Factor (PDGF) Receptor alpha chain (CD140a, PDGFR-a), a receptor tyrosine kinase which is widely expressed on cells of mesenchymal origin in the embryo and adult and on several other cell types during embryonic development, but not on hematopoietic cells. PDGFR-a binds to PDGF A and B chains, in contrast to PDGFR-b, which binds only to the PDGF B chain. Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Binding to PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization. The mAb APA5 has been demonstrated to block binding of PDGF-AA to PDGFR-a-expressing cells in vitro and to block some PDGF-mediated developmental events in vivo.
Synonyms: PDGF Receptor alpha chain
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling|
Application DetailsProduct Details anti-PDGFRA Antibody Target Details PDGFRA Handling References for anti-PDGFRA antibody (ABIN967626) Images back to top
|Application Notes||This antibody has been tested by immunofluorescent staining (Less or equal than 1 µg/million cells) with flow cytometric analysis and immunohistochemical staining of acetone-fixed frozen sections (0.5 - 2.5 µg/ml) to assure specificity and reactivity. Other reported applications include western blot analysis, ELISA, immunohistochemical staining of paraffin-embedded sections, inhibition of ligand binding, and in vivo blocking of PDGF action.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDGFRA Antibody Target Details PDGFRA Application Details References for anti-PDGFRA antibody (ABIN967626) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-PDGFRA antibody (ABIN967626)Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Handling Images back to top
|Product cited in:||
Fruttiger, Calver, Krüger, Mudhar, Michalovich, Takakura, Nishikawa, Richardson: "PDGF mediates a neuron-astrocyte interaction in the developing retina." in: Neuron, Vol. 17, Issue 6, pp. 1117-31, 1997
Takakura, Yoshida, Kunisada, Nishikawa, Nishikawa: "Involvement of platelet-derived growth factor receptor-alpha in hair canal formation." in: The Journal of investigative dermatology, Vol. 107, Issue 5, pp. 770-7, 1996
Heldin: "Structural and functional studies on platelet-derived growth factor." in: The EMBO journal, Vol. 11, Issue 12, pp. 4251-9, 1992