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anti-Human Phospholipase C gamma 2 antibody for Intracellular Staining

Recommended Phospholipase C gamma 2 Antibody (supplied by: Log in to see )

Antigen
Phospholipase C gamma 2 (PLCG2) Antibodies
  • APLAID
  • FCAS3
  • Plcg-2
  • PLCgamma2
Epitope
pTyr759
41
31
21
19
13
5
5
4
4
3
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
193
118
114
6
Host
Mouse
188
5
Clonality
Monoclonal
Conjugate
This Phospholipase C gamma 2 antibody is conjugated to Alexa Fluor 647
4
3
3
3
3
3
3
3
3
3
3
3
1
Application
Intracellular Staining (ICS)
116
72
34
34
28
28
14
5
2
2
1
1
Supplier
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Catalog No. ABIN1177150
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008675529 ABIN1177149 ICS PE Mouse IgG1, kappa pTyr759 Log in to see K86-689-37 1
0.0008675529 ABIN1177151 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr759 Log in to see K86-689-37

Top referenced anti-Phospholipase C gamma 2 antibody for Intracellular Staining

Similar anti-Phospholipase C gamma 2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 121 Antibodies 66 Antibodies 64 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 15 Antibodies 5 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies 19 Antibodies 19 Antibodies
Immunohistochemistry (IHC) 77 Antibodies 46 Antibodies 44 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies 34 Antibodies 34 Antibodies

General

Antigen Phospholipase C gamma 2 (PLCG2) Antibodies
Epitope pTyr759
(41), (31), (21), (19), (13), (5), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(193), (118), (114), (6)
Host Mouse
(188), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Phospholipase C gamma 2 antibody is conjugated to Alexa Fluor 647
(4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Intracellular Staining (ICS)
(116), (72), (34), (34), (28), (28), (14), (5), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-Phospholipase C gamma 2 Antibody

Target Details Phospholipase C gamma 2 Application Details Handling References for anti-Phospholipase C gamma 2 Antibody (ABIN1177150) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human PLC-gamma2 Peptide
Clone K86-689-37
Isotype IgG1, kappa

Target Details Phospholipase C gamma 2

Product Details anti-Phospholipase C gamma 2 Antibody Application Details Handling References for anti-Phospholipase C gamma 2 Antibody (ABIN1177150) Images back to top
Antigen
Alternative Name PLC-gamma 2 (PLCG2 Antibody Abstract)
Background The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLC-gamma is the only member that contains SH2 and SH3 domains. These domains enable it to interact with protein tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: PLC-g1, which is ubiquitously expressed, and PLC-g2, which is found primarily in the hematopoietic system. PLC-g2-null mice exhibit impaired B lymphocyte maturation and defects in Fc receptor functions. Phosphorylation of PLC-g2 at tyrosines 753 and 759 (Y759) is required for activation of PLC-g2 enzyme activity. PLC-g2 phosphorylation at Y759 can be induced by stimulation of the B cell receptor in Ramos cells, the collagen receptor in platelets, and the T cell receptor in Jurkat cells, and it occurs downstream of Btk and BLNK in the signaling cascade of activated B cells. The K86-689.37.73 antibody recognizes PLC-g2 phosphorylated at Y759 in the SH2-SH3 linker region.
Pathways RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-Phospholipase C gamma 2 Antibody Target Details Phospholipase C gamma 2 Handling References for anti-Phospholipase C gamma 2 Antibody (ABIN1177150) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-Phospholipase C gamma 2 Antibody Target Details Phospholipase C gamma 2 Application Details References for anti-Phospholipase C gamma 2 Antibody (ABIN1177150) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-Phospholipase C gamma 2 Antibody (ABIN1177150)

Product Details anti-Phospholipase C gamma 2 Antibody Target Details Phospholipase C gamma 2 Application Details Handling Images back to top
Product cited in:

Holodick, Tumang, Rothstein: "Continual signaling is responsible for constitutive ERK phosphorylation in B-1a cells." in: Molecular immunology, Vol. 46, Issue 15, pp. 3029-36, 2009