anti-Rat (Rattus) Phospholipase C gamma 2 antibody for Intracellular Staining

Recommended Phospholipase C gamma 2 Antibody (supplied by: Log in to see )

Antigen
Phospholipase C gamma 2 (PLCG2) Antibodies
  • PLCgamma2
  • Plcg-2
  • APLAID
  • FCAS3
  • phospholipase C, gamma 2
  • phospholipase C, gamma 2 (phosphatidylinositol-specific)
  • Plcg2
  • PLCG2
Epitope
pTyr759
46
46
39
22
15
5
5
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
314
156
147
12
4
4
4
4
3
1
Host
Mouse
217
97
Clonality
Monoclonal
Conjugate
This Phospholipase C gamma 2 antibody is conjugated to PE
9
9
8
8
8
5
5
5
5
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
Application
Intracellular Staining (ICS)
224
110
86
59
54
46
40
27
16
4
3
2
2
1
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Catalog No. ABIN1177149
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177150 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr759 Log in to see K86-689-37 1
1 ABIN1177151 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr759 Log in to see K86-689-37

General

Antigen Phospholipase C gamma 2 (PLCG2) Antibodies
Epitope pTyr759
(46), (46), (39), (22), (15), (5), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(314), (156), (147), (12), (4), (4), (4), (4), (3), (1)
Host Mouse
(217), (97)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Phospholipase C gamma 2 antibody is conjugated to PE
(9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Intracellular Staining (ICS)
(224), (110), (86), (59), (54), (46), (40), (27), (16), (4), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-Phospholipase C gamma 2 Antibody

Target Details Phospholipase C gamma 2 Application Details Handling ProductDetails: References for anti-Phospholipase C gamma 2 antibody (ABIN1177149) Images
Brand BD Phosflow™
Purification Purified from tissue culture supernatant or ascites by affinity chromatography
Immunogen Phosphorylated Human PLC-gamma2 Peptide
Clone K86-689-37
Isotype IgG1, kappa

Target Details Phospholipase C gamma 2

Product Details anti-Phospholipase C gamma 2 Antibody Application Details Handling ProductDetails: References for anti-Phospholipase C gamma 2 antibody (ABIN1177149) Images back to top
Antigen
Alternative Name PLC-gamma 2 (PLCG2 Antibody Abstract)
Background The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLC-gamma is the only member that contains SH2 and SH3 domains. These domains enable it to interact with protein tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: PLC-g1, which is ubiquitously expressed, and PLC-g2, which is found primarily in the hematopoietic system. PLC-g2-null mice exhibit impaired B lymphocyte maturation and defects in Fc receptor functions. Phosphorylation of PLC-g2 at tyrosines 753 and 759 (Y759) is required for activation of PLC-g2 enzyme activity. PLC-g2 phosphorylation at Y759 can be induced by stimulation of the B cell receptor in Ramos cells, the collagen receptor in platelets, and the T cell receptor in Jurkat cells, and it occurs downstream of Btk and BLNK in the signaling cascade of activated B cells. The K86-689.37.73 antibody recognizes PLC-g2 phosphorylated at Y759 in the SH2-SH3 linker region.
Pathways RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling

Application Details

Product Details anti-Phospholipase C gamma 2 Antibody Target Details Phospholipase C gamma 2 Handling ProductDetails: References for anti-Phospholipase C gamma 2 antibody (ABIN1177149) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-Phospholipase C gamma 2 Antibody Target Details Phospholipase C gamma 2 Application Details ProductDetails: References for anti-Phospholipase C gamma 2 antibody (ABIN1177149) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

ProductDetails: References for anti-Phospholipase C gamma 2 antibody (ABIN1177149)

Product Details anti-Phospholipase C gamma 2 Antibody Target Details Phospholipase C gamma 2 Application Details Handling Images back to top
Product cited in:

Kim, Sekiya, Poulin, Bae, Rhee: "Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 9986-99, 2004