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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||23 Antibodies||32 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||6 Antibodies|
|Immunofluorescence (IF)||18 Antibodies||35 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||56 Antibodies||56 Antibodies|
|Immunohistochemistry (IHC)||46 Antibodies||50 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||24 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||79 Antibodies|
|Antigen||SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SHC1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-SHC1 AntibodyTarget Details SHC1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Shc protein.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
Target Details SHC1Product Details anti-SHC1 Antibody Application Details Handling Images back to top
|Alternative Name||SHC1 / SHC (SHC1 Antibody Abstract)|
|Background||Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus activating mitogenic, as well as other, responses. Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1|
|Molecular Weight||approx. 46,52,66 kDa|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-SHC1 Antibody Target Details SHC1 Handling Images back to top
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoprecipitation: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling back to top