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anti-Mouse (Murine) SHC1 antibody for Immunohistochemistry (Frozen Sections)

Recommended SHC1 Antibody (supplied by: Log in to see )

Antigen
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
  • p66
  • p66shc
  • P66shc
  • shc
  • SHC
  • Shc
  • SHC1
  • ShcA
  • SHCA
  • si:dkey-246i14.4
  • wu:fc10h03
Epitope
C-Term
41
39
17
15
13
13
13
10
9
9
8
8
7
6
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
216
170
132
10
6
6
2
2
1
1
1
Host
Mouse
203
24
Clonality
Monoclonal
Conjugate
This SHC1 antibody is un-conjugated
7
7
6
5
5
5
5
5
5
5
5
5
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
166
78
59
56
49
33
8
6
6
4
3
1
1
Supplier
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Supplier Product No.
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Catalog No. ABIN126887
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN126889 IHC (fro) WB Mouse IgG1 pTyr317 Log in to see 1-50E-012
0.00088051456 ABIN126888 EIA IHC (fro) WB Mouse IgG1 pTyr239, pTyr240 Log in to see 1-00E-003
0.00088051456 ABIN3043923 IHC (fro) IHC (p) WB Rabbit IgG AA 536-564, C-Term Log in to see Polyclonal
0.00088051456 ABIN3032555 ICC IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-SHC1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 32 Antibodies 42 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 7 Antibodies
Immunofluorescence (IF) 34 Antibodies 61 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies 56 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 113 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
Epitope C-Term
(41), (39), (17), (15), (13), (13), (13), (10), (9), (9), (8), (8), (7), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(216), (170), (132), (10), (6), (6), (2), (2), (1), (1), (1)
Host Mouse
(203), (24)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SHC1 antibody is un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(166), (78), (59), (56), (49), (33), (8), (6), (6), (4), (3), (1), (1)
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Product Details anti-SHC1 Antibody

Target Details SHC1 Application Details Handling Images
Specificity This antibody specifically recognizes the C-terminus of shc.
Characteristics Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3,SHCA, Src homology 2 domain-containing-transforming protein C1
Purification Subsequent thiophilic adsorption and size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic peptide conjugated to hemocyanin
Clone 11F6
Isotype IgG1

Target Details SHC1

Product Details anti-SHC1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SHC1 / SHC (SHC1 Antibody Abstract)
Background Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1
Gene ID 6464
UniProt P29353
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SHC1 Antibody Target Details SHC1 Handling Images back to top
Application Notes Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Recommended positive control: Cell lysate from untreated 3T3 cells. Immunocytochemistry. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mLPBS / 0.09 % Na-azide / PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Expiry Date 12 months

Images

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (C-Term) (ABIN126887) anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (C-Term) antibody