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anti-Rat (Rattus) SHC1 antibody for Western Blotting

Recommended SHC1 Antibody (supplied by: Log in to see )

Antigen
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
  • p66
  • p66shc
  • P66shc
  • shc
  • SHC
  • Shc
  • SHC1
  • ShcA
  • SHCA
  • si:dkey-246i14.4
  • wu:fc10h03
Epitope
C-Term
41
39
17
15
13
13
13
10
9
9
8
8
7
6
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
222
173
131
9
5
5
2
1
1
1
Host
Rabbit
207
27
Clonality
Polyclonal
Conjugate
This SHC1 antibody is un-conjugated
7
7
6
5
5
5
5
5
5
5
5
5
1
1
1
Application
Western Blotting (WB)
172
83
62
56
49
38
8
8
7
4
3
1
1
Supplier
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Catalog No. ABIN2784202
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN968228 IF WB Mouse IgG1 AA 359-473 Log in to see 30-SHC 5
0.13476625 ABIN968229 IF WB Mouse IgG1 AA 359-473 Log in to see 30-SHC 5
0.13476625 ABIN126890 WB Mouse IgG1 N-Term Log in to see 2-40E-005
0.13476625 ABIN1531193 IHC ELISA WB Rabbit IgG pTyr349 Log in to see Polyclonal
0.13476625 ABIN1531194 IHC ELISA WB Rabbit IgG pTyr427 Log in to see Polyclonal
0.13476625 ABIN3043923 IHC (fro) IHC (p) WB Rabbit IgG AA 536-564, C-Term Log in to see Polyclonal
0.13476625 ABIN967733 IF IP WB Rabbit Ig Log in to see Polyclonal 5
0.13476625 ABIN967732 IF IP WB Rabbit Ig Log in to see Polyclonal 5
0.13476625 ABIN1532209 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN1532210 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN3044111 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 424-440, C-Term Log in to see Polyclonal
0.13476625 ABIN498175 IHC (p) IP WB Rabbit Log in to see Polyclonal
0.13476625 ABIN257557 ICC IF IHC (p) IHC WB Rabbit IgG Tyr427 Log in to see Polyclonal
0.13476625 ABIN2707047 IHC WB Rabbit pTyr349 Log in to see Polyclonal
0.13476625 ABIN2463337 ELISA WB Rabbit Log in to see Polyclonal
0.13476625 ABIN1870608 IF IHC WB Rabbit IgG pTyr427 Log in to see Polyclonal
0.13476625 ABIN2505399 IHC WB Rabbit IgG Tyr427 Log in to see Polyclonal
0.13476625 ABIN2505400 IHC WB Rabbit IgG pTyr427 Log in to see Polyclonal
0.13476625 ABIN2505404 IF IHC ELISA WB Rabbit IgG Tyr349 Log in to see Polyclonal
0.13476625 ABIN2505402 IHC ELISA WB Rabbit IgG pTyr349 Log in to see Polyclonal

Top referenced anti-SHC1 antibody for Western Blotting

Similar anti-SHC1 Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine) Rat (Rattus) Chicken Cow (Bovine) Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 165 Antibodies 116 Antibodies 10 Antibodies 79 Antibodies 6 Antibodies 5 Antibodies 2 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 11 Antibodies 7 Antibodies 7 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies 27 Antibodies 24 Antibodies
Immunohistochemistry (IHC) 86 Antibodies

General

Antigen SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
Epitope C-Term
(41), (39), (17), (15), (13), (13), (13), (10), (9), (9), (8), (8), (7), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(222), (173), (131), (9), (5), (5), (2), (1), (1), (1)
Host Rabbit
(207), (27)
Clonality
Polyclonal
Conjugate This SHC1 antibody is un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Western Blotting (WB)
(172), (83), (62), (56), (49), (38), (8), (8), (7), (4), (3), (1), (1)
Pubmed 1 reference available
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Product Details anti-SHC1 Antibody

Target Details SHC1 Application Details Handling References for anti-SHC1 Antibody (ABIN2784202) Images
Blocking Peptide Blocking peptide for this product available: ABIN983706
Sequence AEQLRGEPWF HGKLSRREAE ALLQLNGDFL VRESTTTPGQ YVLTGLQSGQ
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against SHC1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human SHC1

Target Details SHC1

Product Details anti-SHC1 Antibody Application Details Handling References for anti-SHC1 Antibody (ABIN2784202) Images back to top
Antigen
Alternative Name SHC1 (SHC1 Antibody Abstract)
Background SHC1 is a signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.
Molecular Weight 52 kDa
Gene ID 6464
NCBI Accession NP_003020, NM_003029
UniProt P29353
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SHC1 Antibody Target Details SHC1 Handling References for anti-SHC1 Antibody (ABIN2784202) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 474 AA

Restrictions For Research Use only

Handling

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details References for anti-SHC1 Antibody (ABIN2784202) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SHC1 Antibody (ABIN2784202)

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling Images back to top
Background publications

George, Schuller, Harris, Ladbury: "A phosphorylation-dependent gating mechanism controls the SH2 domain interactions of the Shc adaptor protein." in: Journal of molecular biology, Vol. 377, Issue 3, pp. 740-7, 2008

Images

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling References for anti-SHC1 Antibody (ABIN2784202) back to top
Supplier Images
Western Blotting (WB) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (C-Term) (ABIN2784202) WB Suggested Anti-SHC1 Antibody Titration: 0.2-1 ug/ml
Positive Control:...