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|Application / Reactivity||Human|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||32 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies|
|Immunofluorescence (IF)||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||40 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||24 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||135 Antibodies|
|Antigen||tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) Antibodies|
|Epitope||AA 22-760 Alternatives|
|Conjugate||This TIE1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-TIE1 AntibodyTarget Details TIE1 Application Details Handling References for anti-TIE1 Antibody (ABIN4899695) Images
|Specificity||Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50 % cross-reactivity with recombinant mouse Tie-1 is observed and less than 1 % cross-reactivity with recombinant human Tie-2 is observed.|
|Sterility||0.2 μm filtered|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant human Tie-1, Ala22-Gln760, Accession # P35590|
Target Details TIE1Product Details anti-TIE1 Antibody Application Details Handling References for anti-TIE1 Antibody (ABIN4899695) Images back to top
|Alternative Name||Tie-1 (TIE1 Antibody Abstract)|
Application DetailsProduct Details anti-TIE1 Antibody Target Details TIE1 Handling References for anti-TIE1 Antibody (ABIN4899695) Images back to top
|Application Notes||Western Blot 0.1 μg/mL, Flow Cytometry 2.5 μg/10^6 cells, Immunohistochemistry 5-15 μg/mL, CyTOF-ready|
|Restrictions||For Research Use only|
HandlingProduct Details anti-TIE1 Antibody Target Details TIE1 Application Details References for anti-TIE1 Antibody (ABIN4899695) Images back to top
|Reconstitution||Reconstitute at 0.2 mg/mL in sterile PBS.|
|Buffer||0.2 μm filtered solution in PBS with Trehalose.|
|Storage||4 °C/-20 °C/-80 °C|
|Storage Comment||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.|
|Expiry Date||12 months|
References for anti-TIE1 Antibody (ABIN4899695)Product Details anti-TIE1 Antibody Target Details TIE1 Application Details Handling Images back to top
|Product cited in:||
Hakanpaa, Sipila, Leppanen, Gautam, Nurmi, Jacquemet, Eklund, Ivaska, Alitalo, Saharinen: "Endothelial destabilization by angiopoietin-2 via integrin β1 activation." in: Nature communications, Vol. 6, pp. 5962, 2015 Method employed by authors: Immunofluorescence (IF) (Sample species: Human).
Marron, Singh, Tahir, Kavumkal, Kim, Koh, Brindle: "Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2." in: The Journal of biological chemistry, Vol. 282, Issue 42, pp. 30509-17, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Kim, Shin, Kim, Choi, Byun, Jeon, Suh, Kim: "Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells." in: Cardiovascular research, Vol. 72, Issue 3, pp. 394-402, 2006 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Danet, Pan, Luongo, Bonnet, Simon: "Expansion of human SCID-repopulating cells under hypoxic conditions." in: The Journal of clinical investigation, Vol. 112, Issue 1, pp. 126-35, 2003 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).
Marron, Hughes, Edge, Forder, Brindle: "Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39741-6, 2001 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).
ImagesProduct Details anti-TIE1 Antibody Target Details TIE1 Application Details Handling References for anti-TIE1 Antibody (ABIN4899695) back to top