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anti-Mouse (Murine) TYRO3 antibody for Immunofluorescence

Recommended TYRO3 Antibody (supplied by: Log in to see )

Antigen
TYRO3 Protein Tyrosine Kinase (TYRO3) Antibodies
  • AI323366
  • brt
  • Brt
  • BYK
  • byk
  • DKFZp469I0432
  • Dtk
  • dtk
  • Etk-2
  • ksy
  • LOC100231598
  • MGC145734
  • REK
  • Rse
  • RSE
  • rse
  • Sky
  • sky
  • tif
  • Tif
  • TYRO3
  • xksy
  • Xksy
Epitope
AA 100-250
17
13
12
8
7
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
165
52
23
4
1
Host
Rabbit
92
72
7
Clonality
Polyclonal
Conjugate
This TYRO3 antibody is un-conjugated
5
4
4
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
122
65
42
39
22
17
13
12
11
10
6
2
1
Supplier
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Catalog No. ABIN440476
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843398 ABIN4306341 ICC IF WB DyLight 405 Rabbit AA 100-250, Internal Region Log in to see Polyclonal
0.0010843398 ABIN4306348 ICC IF WB Alexa Fluor 405 Rabbit AA 100-250, Internal Region Log in to see Polyclonal
0.0010843398 ABIN4306349 IF WB Alexa Fluor 488 Rabbit AA 100-250, Internal Region Log in to see Polyclonal
0.0010843398 ABIN4306350 ICC IF WB Alexa Fluor 647 Rabbit AA 100-250, Internal Region Log in to see Polyclonal
0.0010843398 ABIN4306351 ICC IF WB Alexa Fluor 700 Rabbit AA 100-250, Internal Region Log in to see Polyclonal
0.0010843398 ABIN1740510 ICC IF WB Rabbit IgG AA 100-250 Log in to see Polyclonal

Similar anti-TYRO3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 7 Antibodies 64 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 14 Antibodies
Immunofluorescence (IF) 7 Antibodies 43 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies 38 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Western Blotting (WB) 36 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 6 Antibodies

General

Antigen TYRO3 Protein Tyrosine Kinase (TYRO3) Antibodies
Epitope AA 100-250
(17), (13), (12), (8), (7), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(165), (52), (23), (4), (1)
Host Rabbit
(92), (72), (7)
Clonality
Polyclonal
Conjugate This TYRO3 antibody is un-conjugated
(5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(122), (65), (42), (39), (22), (17), (13), (12), (11), (10), (6), (2), (1)
Pubmed 1 reference available
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Product Details anti-TYRO3 Antibody

Target Details TYRO3 Application Details Handling References for anti-TYRO3 Antibody (ABIN440476) Images
Purification affinity purified
Immunogen Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). Immunogen sequence has 94% identity to rat

Target Details TYRO3

Product Details anti-TYRO3 Antibody Application Details Handling References for anti-TYRO3 Antibody (ABIN440476) Images back to top
Antigen
Alternative Name Dtk/TYRO3 (TYRO3 Antibody Abstract)
Background Gene Symbol: TYRO3
Gene ID 7301
UniProt Q06418
Research Area Signaling, Tyrosine Kinases
Pathways RTK Signaling

Application Details

Product Details anti-TYRO3 Antibody Target Details TYRO3 Handling References for anti-TYRO3 Antibody (ABIN440476) Images back to top
Application Notes This TYRO3 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~96 kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:200, Western Blot 2 µg/mL
Protocol Protocol specific for TYRO3 Antibody Procedure Guide for NBP1-28635 - Tyro3 AntibodyProcedure Guide for NBP1-28635- Tyro3 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-Tyro3 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.(c) 2009 Novus Biologicals - Tyro3 Antibody - Page 1
Restrictions For Research Use only

Handling

Product Details anti-TYRO3 Antibody Target Details TYRO3 Application Details References for anti-TYRO3 Antibody (ABIN440476) Images back to top
Format Liquid
Concentration 1.29 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.

References for anti-TYRO3 Antibody (ABIN440476)

Product Details anti-TYRO3 Antibody Target Details TYRO3 Application Details Handling Images back to top
Product cited in:

Chan, Carrera Silva, De Kouchkovsky, Joannas, Hao, Hu, Huntsman, Eng, Licona-Limón, Weinstein, Herbert, Craft, Flavell, Repetto, Correale, Burchard, Torgerson, Ghosh, Rothlin: "The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity." in: Science (New York, N.Y.), Vol. 352, Issue 6281, pp. 99-103, 2016 Further details: Flow Cytometry

Images

Product Details anti-TYRO3 Antibody Target Details TYRO3 Application Details Handling References for anti-TYRO3 Antibody (ABIN440476) back to top
Supplier Images
Western Blotting (WB) image for anti-TYRO3 antibody (TYRO3 Protein Tyrosine Kinase) (AA 100-250) (ABIN440476) anti-TYRO3 Protein Tyrosine Kinase (TYRO3) (AA 100-250) antibody