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anti-Mouse (Murine) TYRO3 antibody for Western Blotting

Recommended TYRO3 Antibody (supplied by: Log in to see )

Antigen
TYRO3 Protein Tyrosine Kinase (TYRO3) Antibodies
  • TYRO3
  • brt
  • byk
  • dtk
  • rse
  • sky
  • tif
  • Xksy
  • MGC145734
  • DKFZp469I0432
  • LOC100231598
  • BYK
  • Dtk
  • RSE
  • Sky
  • Tif
  • Brt
  • REK
  • ksy
  • xksy
  • AI323366
  • Etk-2
  • Rse
  • TYRO3 protein tyrosine kinase
  • TYRO3 protein tyrosine kinase 3
  • TYRO3
  • tyro3
  • LOC100231598
  • Tyro3
Epitope
AA 100-250, Internal Region
17
13
12
11
8
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
169
58
23
4
1
Host
Rabbit
98
71
12
Clonality
Polyclonal
Conjugate
This TYRO3 antibody is un-conjugated
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
126
72
47
41
27
22
21
17
11
10
6
2
2
Supplier
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Catalog No. ABIN440474
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590793 ABIN392054 IHC (p) WB Rabbit Ig AA 842-873, C-Term Log in to see Polyclonal
2.9590793 ABIN2500879 IHC ELISA WB Rabbit IgG pTyr681, pTyr749 Log in to see Polyclonal
2.9590793 ABIN2500878 IHC ELISA WB Rabbit IgG Tyr681, Tyr749 Log in to see Polyclonal
2.9590793 ABIN2498213 IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
2.9590793 ABIN4914977 WB Mouse IgG1 kappa Log in to see 1444CT895-86-31
1 ABIN359940 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
1 ABIN493544 EIA WB Rabbit IgG Extracellular Domain Log in to see Polyclonal 3
1 ABIN4900191 ELISA (Capture) FACS IHC WB Rat IgG1 AA 31-418 Log in to see 109646 3
1 ABIN872341 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN906157 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN906162 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2500877 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN4899126 FACS IHC WB Biotin Rat IgG1 AA 1-418 Log in to see 109646
1 ABIN2745175 ELISA WB Mouse IgG1, kappa Log in to see 1444CT895-86-31
1 ABIN4306348 FACS ICC IF WB Alexa Fluor 405 Rabbit AA 100-250, Internal Region Log in to see Polyclonal
1 ABIN4306349 FACS ICC IF WB Alexa Fluor 488 Rabbit AA 100-250, Internal Region Log in to see Polyclonal
1 ABIN4306350 FACS ICC IF WB Alexa Fluor 647 Rabbit AA 100-250, Internal Region Log in to see Polyclonal
1 ABIN4306351 FACS ICC IF WB Alexa Fluor 700 Rabbit AA 100-250, Internal Region Log in to see Polyclonal
1 ABIN4268607 ICC IF WB Biotin Rabbit AA 100-250, Internal Region Log in to see Polyclonal
1 ABIN4268611 FACS ICC IF WB DyLight 650 Rabbit AA 100-250, Internal Region Log in to see Polyclonal

Top referenced anti-TYRO3 antibody for Western Blotting

Similar anti-TYRO3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 7 Antibodies 71 Antibodies
ELISA (Capture) 1 Antibodies 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 6 Antibodies
Flow Cytometry (FACS) 21 Antibodies 17 Antibodies
Immunocytochemistry (ICC) 12 Antibodies 22 Antibodies
Immunofluorescence (IF) 12 Antibodies 48 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Western Blotting (WB) 38 Antibodies

General

Antigen TYRO3 Protein Tyrosine Kinase (TYRO3) Antibodies
Epitope AA 100-250, Internal Region
(17), (13), (12), (11), (8), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(169), (58), (23), (4), (1)
Host Rabbit
(98), (71), (12)
Clonality
Polyclonal
Conjugate This TYRO3 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(126), (72), (47), (41), (27), (22), (21), (17), (11), (10), (6), (2), (2)
Pubmed 1 reference available
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Product Details anti-TYRO3 Antibody

Target Details TYRO3 Application Details Handling References for anti-TYRO3 Antibody (ABIN440474) Images
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). [Swiss-Prot# Q06418]

Target Details TYRO3

Product Details anti-TYRO3 Antibody Application Details Handling References for anti-TYRO3 Antibody (ABIN440474) Images back to top
Antigen
Alternative Name Dtk/TYRO3 (TYRO3 Antibody Abstract)
Background Gene Symbol: TYRO3
Molecular Weight Theoretical MW: 96 kDa
Gene ID 7301
UniProt Q06418
Pathways RTK Signaling

Application Details

Product Details anti-TYRO3 Antibody Target Details TYRO3 Handling References for anti-TYRO3 Antibody (ABIN440474) Images back to top
Application Notes Western Blot 2 μg/mL, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:200This TYRO3 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~96 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW cytometry reported in scientific literature ( PMID 27034374).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for TYRO3 Antibody Procedure Guide for NBP1-28635 - Tyro3 AntibodyProcedure Guide for NBP1-28635- Tyro3 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-Tyro3 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.(c) 2009 Novus Biologicals - Tyro3 Antibody - Page 1
Restrictions For Research Use only

Handling

Product Details anti-TYRO3 Antibody Target Details TYRO3 Application Details References for anti-TYRO3 Antibody (ABIN440474) Images back to top
Format Liquid
Concentration 1.19 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TYRO3 Antibody (ABIN440474)

Product Details anti-TYRO3 Antibody Target Details TYRO3 Application Details Handling Images back to top
Product cited in:

Chan, Carrera Silva, De Kouchkovsky, Joannas, Hao, Hu, Huntsman, Eng, Licona-Limón, Weinstein, Herbert, Craft, Flavell, Repetto, Correale, Burchard, Torgerson, Ghosh, Rothlin: "The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity." in: Science (New York, N.Y.), Vol. 352, Issue 6281, pp. 99-103, 2016 Further details: Flow Cytometry

Images

Product Details anti-TYRO3 Antibody Target Details TYRO3 Application Details Handling References for anti-TYRO3 Antibody (ABIN440474) back to top
Supplier Images
Western Blotting (WB) image for anti-TYRO3 antibody (TYRO3 Protein Tyrosine Kinase) (AA 100-250) (ABIN440474) anti-TYRO3 Protein Tyrosine Kinase (TYRO3) (AA 100-250), (Internal Region) antibody
Immunofluorescence (IF) image for anti-TYRO3 antibody (TYRO3 Protein Tyrosine Kinase) (AA 100-250) (ABIN440474) Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody [NBP1-28635] - Staining of...