ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 150+65 kDa bands observed in Jurkat lysate (calculated MW of 133 kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 60 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human spleen shows nuclear staining. Recommended concentration, 2-5 μg/mL.