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|Application / Reactivity||Rhesus Monkey||Cynomolgus||Baboon|
|Western Blotting (WB)||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||1 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies||3 Antibodies||2 Antibodies|
|Antigen||CD4 Molecule (CD4) Antibodies|
|Reactivity||Baboon, Cynomolgus, Rhesus Monkey Alternatives|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS)
|4 references available|
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN2689220) Images
The L200 antibody reacts with the human form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal human donor peripheral blood lymphocytes. L200 antibody also cross-reacts with a subset of CD3-positive peripheral blood lymphocytes, but not monocytes, of both rhesus and cynomolgus macaque monkeys. Cross-reactivity on both lymphocytes and monocytes (weak) of baboon is also observed. The distribution on lymphocytes is similar for both human and monkey, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of anti-CD4 reactivity on peripheral blood lymphocytes of rhesus macaque (Macaca mulatta) analyzed by flow cytometry
BD Pharmingen™ Purified Mouse Anti-Human CD4 - Purified - Clone L200 - Isotype Mouse IgG1, κ - Reactivity Bab, Cyno, Rhe - 0.1 mg
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN2689220) Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN2689220) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN2689220) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-CD4 Antibody (ABIN2689220)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
|Product cited in:||
Verdier, Aujoulat, Condevaux et al.: "Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys." in: Toxicology, Vol. 105, Issue 1, pp. 81-90, 1996 (PubMed).
Jacobsen, Aasted, Broe et al.: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, 1994 (PubMed).
Powell, McClure, Anderson et al.: "Phenotypic and functional differences in NK and LAK cells in the peripheral blood of sooty mangabeys and rhesus macaques." in: Cellular immunology, Vol. 124, Issue 1, pp. 107-18, 1989 (PubMed).
Indzhiia, Yakovleva, Overbaugh et al.: "Baboon T cell lymphomas expressing the B cell-associated surface proteins CD40 and Bgp95." in: Journal of clinical immunology, Vol. 12, Issue 3, pp. 225-36, 1992 (PubMed).